论文部分内容阅读
目的：了解一个细胞凋亡相关新基因ＴＦＡＲ１９在不同种属间的序列同源性。方法：利用表达序列标签（ｅｘｐｒｅｓｅｄｓｅｑｕｅｎｃｅｔａｇ，ＥＳＴ）拼接技术、ＲＴＰＣＲ、ＤＮＡ序列测定技术及计算机分析技术。结果：首次成功进行了小鼠ＴＦＡＲ１９ｃＤＮＡ编码区的ｃＤＮＡ克隆化和序列分析，发现小鼠和人ＴＦＡＲ１９在核苷酸水平上有８１．４％的同源性，在氨基酸水平上有高达９６％的同源性。功能区分析发现，小鼠ＴＦＡＲ１９ｃＤＮＡ序列含编码１２６个氨基酸的开放性读码框架，含１个可能的ｃＡＭＰ和ｃＧＭＰ依赖的蛋白激酶磷酸化位点，４个可能的ＰＫＣ磷酸化位点。其Ｃ端的“ＥＤＤＡＤＹ”序列与人ＤＮＡ拓扑异构酶Ｉ１４１１４７位残基序列完全相同。结论：小鼠ＴＦＡＲ１９是与人ＴＦＡＲ１９高度同源的新基因，与ＤＮＡ拓扑异构酶Ｉ可能有功能相关性。
OBJECTIVE: To understand the sequence homology of TFAR19, a novel apoptosis-related gene, between different species. Methods: Using expresedsequencetag (EST) splicing technique, RTPCR, DNA sequencing and computer analysis techniques. RESULTS: The cDNA cloning and sequence analysis of the mouse TFAR19 cDNA coding region was successfully performed for the first time. It was found that mouse and human TFAR19 have 81.4% homology at the nucleotide level and up to 96% at the amino acid level Homology. Functional domain analysis found that the mouse TFAR19 cDNA sequence contains an open reading frame of 126 amino acids containing one possible cAMP and cGMP dependent protein kinase phosphorylation sites and four possible PKC phosphorylation sites. Its C-terminal “EDDADY” sequence and human DNA topoisomerase I141 147 residue sequence is exactly the same. CONCLUSION: Mouse TFAR19 is a novel gene highly homologous to human TFAR19 and may be functionally related to DNA topoisomerase I.