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目的:建立用高效液相色谱(HPLC)同时测定紫锥菊地上部分单咖啡酰酒石酸、咖啡酸、绿原酸和菊苣酸4种成分含量的方法。方法:采用HPLC法,色谱柱为ZORBAX SB-C_(18)(250 mm×4.6 mm,5μm);梯度洗脱,流动相为乙腈-0.1%磷酸溶液,流速1.0 m L·min~(-1);柱温35℃,检测波长为330 nm,进样量10μL。结果:4种酚酸类成分分离效果良好,单咖啡酰酒石酸、绿原酸、咖啡酸、菊苣酸分别在4.19~104.80μg/m L,0.81~20.24μg/m L,0.77~19.28μg/m L,13.20~330.00μg·m L~(-1)内线性关系良好;平均加样回收率在97.5%~101.6%。结论:本法准确、灵敏,重现性较好,可实现检控紫锥菊地上部分酚酸类成分的含量。
Objective: To establish a method for the simultaneous determination of four components of monocalciferyl tartrate, caffeic acid, chlorogenic acid and chicory acid in aerial parts of Echinacea purpura by high performance liquid chromatography (HPLC). Methods: The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution with a flow rate of 1.0 mL · min -1 (-1) using a mobile phase of ZORBAX SB-C 18 (250 mm × 4.6 mm, 5 μm) ). The column temperature was 35 ℃, the detection wavelength was 330 nm and the injection volume was 10μL. Results: The results showed that the four phenolic acids had a good separation effect. The contents of monocalcyl tartrate, chlorogenic acid, caffeic acid and chicory acid were in the ranges of 4.19-104.80μg / m L, 0.81-20.24μg / m L, 0.77-19.28μg / m L, the linearity ranged from 13.20 to 330.00 μg · m L -1. The average recoveries ranged from 97.5% to 101.6%. Conclusion: This method is accurate, sensitive and reproducible, and can control the content of phenolic acids in aerial parts of Echinacea purpurea.