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介绍了用抗ECAMcAb双位点夹心ELISA法检测尿中肠杆菌科细胞共同抗原(ECA)快速诊断尿路感染的临床研究。960份送检标本中,肠杆菌科细菌>105CFU/ml的223例,ELISAP/N>3.1的222例,2.2~3.1的1例;细菌在102~105间的86例,P/N>3.1的50例,2.2~3.1的32例,≤2.1的4例;有4例无菌生长尿液,P/N>2.1,抗生素活性均阳性。结果表明,用免疫方法检测ECA不受抗生素等干扰因素影响,有快速诊断尿路感染的价值。
A clinical study on the rapid diagnosis of urinary tract infection by Enterococcus faecalis common antigen (ECA) in urine by anti-ECAMcAb two-site sandwich ELISA was introduced. Of the 960 samples tested, 223 Enterobacteriaceae bacteria> 105 CFU / ml, 222 cases with ELISAP / N> 3.1, and 1 case from 2.2 to 3.1; 86 cases with 102 to 105 bacteria , P / N> 3.1 in 50 cases, 2.2 ~ 3.1 in 32 cases, ≤ 2.1 in 4 cases; 4 cases of sterile growth urine, P / N> 2.1, antibiotic activity Are positive. The results showed that immune method to detect ECA without antibiotics and other interference factors, the rapid diagnosis of urinary tract infection value.