组织培养冷藏这种保存种质的新方法,特别适用于目前尚无有效方法进行长期贮藏的无性繁殖作物。以往,为了保存马铃薯(Solanum tuberosum)种质,采用的是贮藏愈伤组织和分生组织培养的方法。但愈伤组织培养会遭受初生性腐烂,而分生组织培养涉及额外的场地、人力,以及转移中污染的危险。本篇报道了从经冷冻(-196℃)贮藏24个月的分生组织中完全 Tissue culture The new method of conserving germplasm in cold storage is particularly suitable for clonal crops that have not been effectively preserved for long-term storage. In the past, in order to preserve the germplasm of Solanum tuberosum, a method of storing callus and meristematic culture was used. However, callus cultures suffer from primary decay, whereas meristem culture involves additional site, manpower, and the risk of contamination during transfer. This article reports complete from mesenchymal tissue that has been stored frozen for 24 months (-196 ° C)