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将鼻咽癌病人的肿瘤组织移植于无胸腺小鼠,经传代去除了非恶性的细胞成份,染色体检查证明无胸腺小鼠的移植肿瘤来源于人的肿瘤。电镜下发现桥粒和角蛋白纤维,为上皮细胞。细胞内存在 EB 病毒的核抗原。作者将小鼠上生长的鼻咽癌移植瘤在体外培养,用 Eagle 氏培养液辅以非必需氨基酸和20%胎牛血清。上皮细胞在体外生长迅速,可获得纯的单层上皮细胞,表现
The tumor tissue of patients with nasopharyngeal carcinoma was transplanted into athymic mice, and the non-malignant cell components were removed by passage. The chromosome examination showed that the transplanted tumors of athymic mice originated from human tumors. Desmosomes and keratin fibers were found under electron microscopy as epithelial cells. Epstein-Barr virus nuclear antigen present in cells. The mice were grown in vitro transplanted nasopharyngeal carcinoma cultured in vitro, with Eagle’s medium supplemented with non-essential amino acids and 20% fetal bovine serum. Epithelial cells grow rapidly in vitro, can obtain pure monolayer of epithelial cells, performance