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目的:探讨脑灵汤对阿尔茨海默病(AD)模型大鼠海马CA3区APP蛋白表达的影响,并揭示AD发病的部分机制和脑灵汤可能的作用机制。方法:选取40只SPF清洁级SD大鼠,随机分为正常对照组、假手术组、AD组、脑灵汤组和脑复康组,每组8只,喂养1周后,采用Aβ1-42注射大鼠海马法制成大鼠模型,再喂养28 d,治疗后用Morris水迷宫实验检测大鼠学习记忆行为能力,HE染色检测大鼠海马组织形态学变化,免疫组织化学法观察APP蛋白在大鼠海马CA3区中的表达。结果:Morris水迷宫实验结显示:AD大鼠组的隐匿平台逃避潜伏期延长(P<0.05),在探索实验中,AD大鼠存在记忆障碍(P<0.05),而脑灵汤治疗后可使AD大鼠的逃避潜伏期缩短(P<0.05),大鼠平均探索次数明显增多(P<0.05)。AD组大鼠海马CA3区锥体细胞排列紊乱,有细胞缺失的现象,APP蛋白表达增多,脑灵汤治疗后可改善CA3区椎体细胞的病理改变以及降低APP蛋白的表达。结论:脑灵汤能明显改善AD模型大鼠低下的空间学习记忆能力,降低模型大鼠海马CA3区APP表达,对老年性痴呆大鼠学习记忆能力可能有提高作用。
Objective: To investigate the effect of Naoling Decoction on the expression of APP protein in hippocampal CA3 region of Alzheimer’s disease (AD) model rats and to reveal the possible mechanism of action of Naoling decoction. Methods: Forty Sprague-Dawley (SD) SD rats were randomly divided into normal control group, sham operation group, AD group, Naoling decoction group and naofukang group. Each group was fed with Aβ1-42 The rats were injected into the hippocampus of rats for 28 days. The Morris water maze test was used to detect the learning and memory abilities of rats. The morphological changes of hippocampus of rats were observed by HE staining. The expression of APP protein was detected by immunohistochemistry Expression of hippocampal CA3 region in rats. Results: The Morris water maze test showed that the hidden platform of AD rats prolonged the escape latency (P <0.05). In the exploratory experiments, AD rats had memory impairment (P <0.05) The escape latency of AD rats was shortened (P <0.05), and the average number of explorations in rats was significantly increased (P <0.05). The hippocampal CA3 pyramidal cells in AD group were disordered and cells were missing. The expression of APP protein was increased. After treatment with Naoling decoction, the pathological changes of CA3 vertebral cells and the expression of APP protein were decreased. Conclusion: Naoling decoction can significantly improve the spatial learning and memory abilities of rats with AD and reduce the expression of APP in hippocampal CA3 region of AD model rats, and may improve the learning and memory ability of AD rats.