Fruit flies are considered economically important insects due to some species being agricultural pests.However,morphological identification of fruit fly adults and larvae can be difficult requiring a high level of taxonomic expertise,with misidentifications caus-ing problematic false-positive/negative results.While destructive molecular techniques can assist with the identification process,these often cannot be applied where it is mandatory to retain a voucher reference specimen.In this work,we non-destructively(and partial-destructively)processed larvae and adults mostly belonging to the species Dirioxa pornia(Walker,1849),of the poorly studied nonpest fruit fly tribe Acanthonevrini(Tephritidae)from Australia,to enable molecular identifications whilst retaining morphological vouch-ers.By retaining the morphological features of specimens,we confirmed useful characters for genus/species-level identification,contributing to improved accuracy for future diag-nostics using both molecular and morphological approaches.We provide DNA barcode information for three species of Acanthonevrini known from Australia,which prior to our study was only available for a single species,D.pornia.Our specimen examinations provide new distribution records for three nonpest species:Acanthonevroides variegatus Permkam and Hancock,1995 in South Australia,Acanthonevroides basalis(Walker,1853)and D.pornia in Victoria,Australia;as well as new host plant records for D.pornia,from kangaroo apple,apricot and loquat.