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2009年7月在内蒙古黑城子北、多伦县城东和正蓝旗元上都遗址3个样地分别从0-10 cm、10-20 cm、20-30cm、30-40cm和40-50 cm 5个土层采集沙蒿(Artemisia sphaerocephala)根围土壤和根样,系统研究了沙蒿根围AM真菌和DSE(Darkseptate endophytes)的空间分布及与土壤因子的相关性。结果表明,沙蒿根系能被AM真菌高度侵染形成典型的I-型(Intermediate type)丛枝菌根,并发育形成泡囊和丛枝结构,并与DSE形成良好的共生关系,样地生态条件和采样深度对AM真菌分布和活动有显著影响。黑城子样地孢子密度最高,元上都样地泡囊定殖率最高,不同样地间丛枝、菌丝、总定殖率和DSE定殖率无显著差异。孢子密度峰值出现在0-10cm表层土,并随土壤剖面深度增加而降低;泡囊定殖率峰值出现在10-20cm土层;AM真菌其他结构定殖率及DSE定殖率在各土层间差异不显著或变化无规律。孢子密度与AM真菌不同结构定殖率无显著相关性,与各土壤因子极显著正相关。泡囊定殖率与脲酶和碱性磷酸酶极显著负相关,与酸性磷酸酶显著负相关。菌丝定殖率、总定殖率及DSE定殖率与各土壤因子均无显著相关性。土壤碱解N和有机C与脲酶、酸性磷酸酶和碱性磷酸酶极显著正相关;土壤速效P与碱性磷酸酶极显著正相关,与脲酶显著正相关。对沙蒿根系AM真菌和DSE真菌分布和定殖规律的研究,可进一步明确AM真菌和DSE的生态功能,为利用菌根生物技术促进荒漠植被恢复和生态重建提供依据。
In July 2009, the three plots from the north of Hechengzi in Inner Mongolia, the east of Duolun County and the Shangshengdu site of Zhenglan Banner were numbered from 0-10 cm, 10-20 cm, 20-30 cm, 30-40 cm and 40-50 cm 5 respectively The soil samples and root samples of Artemisia sphaerocephala collected from three soil layers were used to systematically study the spatial distribution of AM fungi and DSE (Darkseptate endophytes) and their correlation with soil factors. The results showed that Artemisia ordosica root could be highly infected with AM fungi to form a typical type I arbuscular mycorrhizal and develop into vesicle and bush structure and form a good symbiotic relationship with DSE. Condition and sampling depth have a significant effect on AM fungal distribution and activity. The highest density of spore in HeiChengzi was the same, the highest rate of vesicle colonization was observed in all samples. There was no significant difference in the rate of colonization, mycelium, total colonization rate and DSE colonization rate between different sites. The spore density peak appeared in 0-10 cm topsoil and decreased with the depth of soil profile increasing. The peak value of vesicle colonization rate appeared in 10-20 cm soil layer. The colonization rates of other structures and DSE colonization rate of AM fungi in each soil layer No significant difference between the changes or irregular. There was no significant correlation between the spore density and the colonization rate of AM fungi, which was significantly and positively correlated with the soil factors. The vesicle colonization rate was significantly negatively correlated with urease and alkaline phosphatase and negatively correlated with acid phosphatase. Mycelial colonization rate, total colonization rate and DSE colonization rate had no significant correlation with soil factors. Soil alkalinity N and organic C were significantly and positively correlated with urease, acid phosphatase and alkaline phosphatase. Soil available P had a significant positive correlation with alkaline phosphatase and a significant positive correlation with urease. Studying on the distribution and colonization rules of arbuscular mycorrhizal fungi and DSE fungi can further clarify the ecological functions of AM fungi and DSE and provide the basis for utilizing mycorrhizal biotechnology to promote the recovery of desert vegetation and ecological reconstruction.