目前对物表消毒效果考核（下称“考核物表”），常用的检测方法有载体法、表面涂抹法等，但对物体深层消毒效果的考核未见有关的报道。为了探索一种既能用于考核物表、又能用于深层消毒效果的试验方法，我们采用了单层及双层琼脂倾注平板法计数菌落；并对琼脂平板涂抹法的... At present, the table disinfection effect assessment (hereinafter referred to as “assessment table”), commonly used detection methods are carrier method, the surface smear method, but the deep disinfection of objects no assessment of the impact of the report. In order to explore a test table that can be used both for evaluation of objects, but also for deep disinfection effect, we used a single and double agar pour plate method to count colonies; and agar plate smear method.