论文部分内容阅读
目的本文检测CD4+CD25+Foxp3+调节性T细胞在子痫前期患者外周血及胎盘附着处蜕膜中的表达,探讨其在子痫前期免疫耐受失衡中的作用。方法选择子痫前期患者20例,正常晚期妊娠患者20例。采用流式细胞仪检测外周血CD4+CD25+Foxp3+的表达;免疫组织化学法检测蜕膜CD4+CD25+调节性T细胞的特异性转录因子Foxp3的表达。结果 1.子痫前期组外周血CD4+CD25+Foxp3+T细胞表达率(1.70±0.23%)明显低于正常晚期妊娠对照组(3.55±0.47%)(P<0.05)。2.蜕膜中Foxp3在子痫前期组的表达阳性率为(16.67%)明显低于正常对照组(66.67%)(P<0.05)。结论子痫前期患者外周血和蜕膜组织中的CD4+CD25+Foxp3+调节性T细胞均低于正常孕妇,提示其数量的减少使其免疫抑制功能减弱,母胎免疫耐受失衡,导致子痫前期的发生。
Objective To detect the expression of CD4 + CD25 + Foxp3 + regulatory T cells in the decidua of peripheral blood and placenta attached to preeclampsia and to explore its role in the imbalance of immune tolerance in preeclampsia. Methods 20 cases of preeclampsia and 20 cases of normal late pregnancy were selected. The expression of CD4 + CD25 + Foxp3 + was detected by flow cytometry. The expression of Foxp3, a specific transcription factor of decidual CD4 + CD25 + regulatory T cells, was detected by immunohistochemistry. The expression of CD4 + CD25 + Foxp3 + T cells in peripheral blood of preeclampsia group (1.70 ± 0.23%) was significantly lower than that of normal late pregnancy group (3.55 ± 0.47%) (P <0.05). The positive rate of Foxp3 expression in preeclampsia group (16.67%) was significantly lower than that in normal control group (66.67%) (P <0.05). Conclusion The CD4 + CD25 + Foxp3 + regulatory T cells in peripheral blood and decidua of preeclampsia patients are lower than that of normal pregnant women, suggesting that the decrease of their numbers weakens the immunosuppressive function and the imbalance of immunotolerance in maternal fetuses, resulting in the progression of preeclampsia happened.