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昆虫病毒的经卵传递,对于昆虫种群中病毒病的自然发生和传播有着重要意义,有关这方面的工作,前人已有一些报道,作者在利用质型多角体病毒(CPV)防治赤松毛虫工作中,对赤松毛虫CPV经卵传递的现象进行了观察,报道如后。 材料和方法 CPV系中国林科院林科所提供。将CPV悬液浸湿松枝,室内晾干后饲喂6—7龄幼虫,室外选择虫口密度较大的林地,在幼虫6—7龄时用CPV悬液喷雾防治。分别收取室内和防治区松树上的虫蛹,单个置于灭菌玻璃容器中,成虫羽化后,检查蛹便有无多角体。解剖部分成虫观察肠道病变,检查中肠有无多角体,另部分成虫置养虫笼中产卵,幼虫孵化后,单个饲养于灭菌容器中,逐日检查记载子代病死虫数。用扫描电镜检查雌蛾外生殖器和卵的表面有无多角体。
The transmissibility of insect viruses is of great significance to the natural occurrence and spread of viral diseases in insect populations. Some reports have been made on the work in this field. The author is using the CPV to prevent and control Civetfly In the red caterpillar Demodex CPV by the phenomenon of egg transmission was observed, reported as after. Materials and Methods CPV was provided by the Chinese Academy of Forestry Linke. The CPV suspension was soaked in pine branches, 6-7 instars of larvae were fed after indoor airing, and the larvae of 6-7 instar larvae were sprayed with CPV suspension to control the density of larvae. Separately collect indoor and pine pest control zone, a single placed in sterile glass containers, adults emerge, check whether the polygons pupae. Anatomical part of the adult observation of intestinal lesions, check whether the polygodal midgut, and the other part of the adult worms spawning cages, the larvae hatch, a single reared in sterile containers, check the generation of dead insects on a daily basis. Using scanning electron microscopy female moth external genitalia and egg surface with or without polyhedron.