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目的:建立一种基于大分子物质环糊精的共振散射光色谱差异测定牛奶中残余黄曲霉毒素M l的新型检测方法。方法:β-环糊精与黄曲霉毒素M l结合后,不同浓度黄曲霉毒素M l与环糊精形成的包合物存在不同强度的共振散射光光谱,用共振瑞利散射光对黄曲霉毒素M l进行检测。结果:在波长为335 nm处共振光散射增强强度与黄曲霉毒素M l的浓度呈线性关系。在pH=4.0~5.0时,包合物形成的最佳温度为60℃,静置时间为1 h,方法的检出限为0.38 ng/ml;线性范围为0 ng/ml~8.0 ng/ml,线性相关系数r=0.992回收率为85%~108%。结论:本方法可简便,快速地测定牛奶中的黄曲霉毒素M l,结果可靠。
OBJECTIVE: To establish a novel method for the determination of residual aflatoxin M 1 in milk based on the difference of resonance light scattering spectra of macromolecular cyclodextrins. Methods: After the binding of β-cyclodextrin and aflatoxin M l, different intensities of the inclusion complex of aflatoxin M l and cyclodextrins were detected by resonance scattering light spectroscopy. Resonance Rayleigh scattering light Toxin M l for testing. Results: The intensity of resonance light scattering at a wavelength of 335 nm showed a linear relationship with the concentration of aflatoxin M1. The optimal temperature for the inclusion complex formation was 60 ℃ and the settling time was 1 h at pH = 4.0-5.0. The detection limit was 0.38 ng / ml. The linear range was 0 ng / ml ~ 8.0 ng / ml , Linear correlation coefficient r = 0.992 recovery rate of 85% to 108%. Conclusion: This method can be used to determine aflatoxin M1 in milk conveniently and rapidly, and the results are reliable.