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本文用四血管闭塞法制成大鼠脑缺血及缺血后再灌流模型,测定脑缺血期及再灌流期不同时间的脑组织丙二醛(MDA)及低分子量铁复合物(LMWS-Fe)含量,并观察去铁胺和异搏定对大鼠脑MDA含量及EEG的影响。结果显示:单纯脑缺血大鼠的脑MDA含量无增高;脑缺血15分钟再通30分钟后,MDA显著增高(P<0.01),并达到高峰;再通60分钟后,MDA仍维持于较高水平(P<0.01);再通120分钟后,MDA降至对照水平(P>0.05)。脑缺血期及再灌流期均未发现脑LMWS-Fe含量升高。再灌流期给予去铁胺或/和异搏定均不能抑制脑脂质过氧化的发生,亦不能改善EEG。
In this study, a rat model of cerebral ischemia and reperfusion after ischemia was established by four-vessel occlusion method. Malondialdehyde (MDA) and low molecular weight iron complex (LMWS-Fe ) Content, and observed deferoxamine and verapamil on brain MDA content and EEG. The results showed that there was no increase of MDA content in brain of rats with cerebral ischemia; MDA increased significantly (P <0.01) 30 min after reperfusion for 15 minutes and peaked at 60 minutes; (P <0.01). After 120 minutes of reperfusion, MDA decreased to the level of control (P> 0.05). Cerebral ischemia and reperfusion period did not find the brain LMWS-Fe increased. Reperfusion period given deferoxamine or / and verapamil can not inhibit the occurrence of cerebral lipid peroxidation, also can not improve EEG.