论文部分内容阅读
目的研究维生素K2(VK2)对NB4细胞生长、凋亡、周期阻滞及分化的诱导作用。方法通过四唑盐比色法、形态学观察、流式细胞仪检测细胞早期凋亡率、细胞周期分析、NBT还原法和流式检测CD14、CD11b阳性率研究维生素K2对NB4细胞的影响。结果NB4细胞经VK2作用后增殖受到抑制;VK2作用NB4细胞72h后,细胞形态呈现凋亡特征,细胞早期凋亡率随浓度升高而升高,与对照组比较及两两比较P<0.01;各药加组G0/G1期细胞均较空白对照组升高,各加药组与空白对照组比较P<0.01,但各加药组之间两两比较无统计学意义;各加药组NBT阳性率、CD14、CD11b表达均较空白对照组无统计学意义。结论VK2对NB4细胞有生长抑制作用,且具有时间及剂量依赖性;VK2可诱导NB4细胞凋亡,且呈剂量依赖性;VK2可诱导NB4细胞周期受阻于G0/G1期,但细胞周期阻滞作用无量效关系;VK2无单独诱导NB4细胞分化作用。
Objective To investigate the induction of vitamin K2 (VK2) on the growth, apoptosis, cell cycle arrest and differentiation of NB4 cells. Methods The effects of vitamin K2 on NB4 cells were studied by tetrazolium salt colorimetric method, morphological observation and flow cytometry. Cell apoptosis rate, cell cycle analysis, NBT reduction and flow cytometry were used to detect the positive rates of CD14 and CD11b. Results After NB4 cells were treated with VK2, the proliferation of NB4 cells was inhibited. After 72 hours VK2 cells were treated with VK2, the cell morphology was characterized by apoptosis. The apoptosis rate of NB4 cells increased with the increase of concentration. Compared with the control group, Each drug plus group G0 / G1 phase cells were higher than the blank control group, each dosing group compared with the blank control group P <0.01, but no significant difference between the two groups dosing group; NBT Positive rate, CD14, CD11b expression than the blank control group was not statistically significant. Conclusion VK2 can inhibit the growth of NB4 cells in a time-and dose-dependent manner. VK2 can induce the apoptosis of NB4 cells in a dose-dependent manner. VK2 induces NB4 cell cycle arrest in G0 / G1 phase, but cell cycle arrest VK2 alone did not induce NB4 cell differentiation.