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目的:分析不同E型肉毒梭菌的肉毒神经毒素(BoNT)基因序列的同源性,预测E型BoNT的B细胞表位。方法:用LaserGene软件中的M egA lign程序比对GenBank中5株不同E型肉毒梭菌的BoNT基因序列;分别利用B ioSun和LaserGene软件分析E型BoNT的表位参数、蛋白质二级结构及氨基酸序列保守性,利用三维结构显示软件Cn3D,分析E型BoNT可能的B细胞表位的空间定位,进而预测E型BoNT的B细胞表位。结果:5个E型BoNT基因核酸序列同源性为97.2%~100%,氨基酸序列同源性为95%~100%;E型BoNT轻链中的62~78,111~127区段,重链中443~465,661~677,693~709,727~743,853~869,1 093~1 109,1 128~1 144,1 163~1 179区段最有可能是其B细胞优势表位。结论:我们利用不同分析软件,结合多参数综合预测出了E型BoNT的多个B细胞表位,为进一步鉴定E型BoNT的B细胞表位及其多表位疫苗的研究奠定了基础。
OBJECTIVE: To analyze the homology of the botulinum neurotoxin (BoNT) gene sequences of different Clostridium botulinum type E and predict the B cell epitopes of E-type BoNT. Methods: The BoNT gene sequences of five strains of Clostridium botulinum type E were genotyped in GenBank by using M egA lign program in LaserGene software. The epitopes of E-type BoNT were analyzed by B ioSun and LaserGene respectively. Amino acid sequence conservation, the use of three-dimensional structure software Cn3D analysis of E-type BoNT possible B cell epitopes in the spatial location, and then predict the B-cell epitope E-BoNT. Results: The homology of five E type BoNT genes was 97.2% ~ 100%, and the homology of amino acid sequence was 95% ~ 100%. The region of 62 ~ 78,111 ~ 127 in E type BoNT light chain, 443 ~ 465,661 ~ 677,693 ~ 709,727 ~ 743,853 ~ 869,1 093 ~ 1 109,1 128 ~ 1 144,1 163 ~ 1 179 The segment is most likely the dominant B cell epitopes. CONCLUSIONS: We predicted multiple B cell epitopes of BoNT with E-type using different analysis softwares combined with multi-parameter synthesis, which laid the foundation for the further identification of B cell epitopes of E-BoNT and multi-epitope vaccines.