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目的建立一种理想的猕猴局灶性脑缺血再灌注模型。方法成年健康猕猴12只(雌雄各半)。经颈总动脉或股动脉介入手术,将标准微球囊导管插入大脑中动脉(MCA)的起始部,然后充盈微球囊阻断MCA血流,退出微球囊后实现MCA血流再灌注,建立大脑中动脉闭塞再灌注(MCAO/R)模型。通过脑血管造影、磁共振血管成像(MRA)、磁共振扫描成像(MRI)、氯化三苯基四氮唑(TTC)染色和神经行为功能评分对动物模型进行评价。结果经颈总动脉或股动脉介入手术,可以在荧光屏直视下准确地将微球囊导管插入大MCA阻断其血流,MCA在MRA上不显像。MCA供血区磁共振T1、T2、DWI出现高信号区,TTC染色显示脑梗死病灶,动物出现相应的神经功能障碍。该方法成功率高、重复性好、操作简单。结论经股动脉微球囊导管介入手术建立猕猴MCAO/R模型是一种较为理想的方法。
Objective To establish an ideal model of focal cerebral ischemia-reperfusion in macaque. Methods 12 adult healthy macaques (half male and half female). Through the common carotid artery or femoral artery interventional surgery, the standard microcapsule catheter was inserted into the beginning of the middle cerebral artery (MCA), then filled the microspheres to block the MCA blood flow, and then out of the microcapsules to achieve MCA blood flow reperfusion , Establishing middle cerebral artery occlusion reperfusion (MCAO / R) model. Animal models were evaluated by cerebrovascular angiography, magnetic resonance angiography (MRA), magnetic resonance imaging (MRI), triphenyltetrazolium chloride (TTC) staining and neurobehavioral functional scores. Results The intervention of the common carotid artery or femoral artery could accurately insert the micro-balloon catheter into the large MCA under the direct view of the fluorescent screen to block the blood flow. The MCA did not show on the MRA. Magnetic resonance MRA T1, T2, DWI high signal area, TTC staining showed cerebral infarction, animals showed the corresponding neurological dysfunction. The method has high success rate, good repeatability and simple operation. Conclusion The establishment of MCAO / R model of cynomolgus monkey by micro-balloon catheterization via femoral artery is an ideal method.