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采用硫代巴比妥酸(TBA)显色法测定油酸致急性肺损伤后血浆及肺组织脂质过氧化物(LPO)的代谢产物丙二醛(MDA)的含量,用薄层层析及无机磷定量法测定肺表面活性物质(PS)的主要功能成分—卵磷脂(PC)和总磷脂(TPL)含量。观察到油酸致肺损伤后血浆和肺组织匀浆MDA含量明显增加;肺组织匀浆PC及TPL含量明显减少。损伤前给予645—2.可减少LPO的生成,且能防止PS的减少。结果提示PS减少与脂质成分自身变化有关,可能是LPO增加致PS合成减少。654—2能抑制这一过程。
The content of malondialdehyde (MDA), a metabolite of lipid peroxide (LPO) in plasma and lung tissue after oleic acid-induced acute lung injury was determined by thiobarbituric acid (TBA) chromogenic assay. And inorganic phosphorus determination of pulmonary surfactant (PS) the main functional components - lecithin (PC) and total phospholipid (TPL) content. The MDA content in plasma and lung homogenates was significantly increased after oleic acid-induced lung injury; the content of PC and TPL in lung homogenate was significantly reduced. Administration of 645-2 before injury can reduce LPO production and prevent the reduction of PS. The results suggest that the decrease in PS is related to changes in the lipid composition itself, which may be due to an increase in LPO and a decrease in PS synthesis. 654-2 can inhibit this process.