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目的研究中链甘油三酯(MCT)在高热高湿条件下抗疲劳的效果和机制。方法将60只雄性ICR小鼠随机分为5组:常温对照组(CC组)、高温对照组(GC组)、运动对照组(SC组)、MCT对照组(MC组)和MCT训练组(MS组)。GC、SC、MC、MS各组每日在温度(36±2)℃,湿度(60±3)%的环境下进行热习服30 min,习服后在相同环境下进行转棒训练30 min。21 d后,各组取6只小鼠在温度(38±2)℃,湿度(60±3)%)环境下,测定转棒力竭时间。SC组、MS组取其余6只小鼠转棒运动30 min后,取血、肝脏、骨骼肌,CC组、SC组、MC组直接取材,并测定血糖、甘油三酯、血清乳酸脱氢酶、肌酸激酶、肝脏糖原、游离脂肪酸含量,以及股四头肌PGC-1α的表达。结果 MCT和运动可以显著延长小鼠在高温高湿环境下转棒力竭时间(P<0.01),与SC组相比,显著降低乳酸脱氢酶、肌酸激酶、血糖和游离脂肪酸(P<0.05),增加肝糖原含量(P<0.05),上调PCG-1α(P<0.05)。结论 MCT可显著提高小鼠在高温高湿环境下抗疲劳能力,其机制与MCT增加骨骼肌代谢调节因子表达,调节糖脂代谢,从而提高运动耐力有关。
Objective To study the anti-fatigue effect and mechanism of medium-chain triglyceride (MCT) under high temperature and high humidity conditions. Methods Sixty male ICR mice were randomly divided into 5 groups: normal control group (CC group), high temperature control group (GC group), exercise control group (SC group), MCT control group (MC group) and MCT training group MS group). The GC, SC, MC and MS groups were subjected to hot acclimation for 30 min daily under the conditions of temperature (36 ± 2) ℃, humidity (60 ± 3)%, . After 21 d, 6 mice in each group were tested for rod exhaust time under the conditions of temperature (38 ± 2) ℃, humidity (60 ± 3)%). The remaining 6 mice in SC group and MS group were taken out of the blood, liver, skeletal muscle, CC group, SC group and MC group directly after 30 min of exercise. The blood glucose, triglyceride, serum lactate dehydrogenase , Creatine kinase, liver glycogen, free fatty acid content, and quadriceps PGC-1α expression. Results Compared with SC group, MCT and exercise significantly prolonged the exhaustion time (P <0.01) of the rods in the rats under high temperature and high humidity, and significantly decreased lactate dehydrogenase, creatine kinase, blood glucose and free fatty acids (P < 0.05), increased hepatic glycogen content (P <0.05) and up-regulated PCG-1α (P <0.05). Conclusion MCT can significantly increase the anti-fatigue ability of mice under high temperature and high humidity. The mechanism is related to MCT increasing skeletal muscle metabolic regulator expression, regulating glucose and lipid metabolism, thereby improving exercise tolerance.