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目的:通过多位点可变数目串联重复序列(MLVA)分型,了解甘肃省临床分离结核分枝杆菌菌株基因型情况。方法:选择标化的15个VNTR位点,对临床分离菌株DNA进行检测,DNA指纹图谱使用BioNumerics4.5软件进行统计分析,得出聚类分析结果。结果:228株结核分枝杆菌被分为4大基因群,7个主要基因型,分别包含13(5.7%)、3(1.3%)、7(3.1%)、1(0.4%)、171(75.0%)、31(13.6%)、2(0.9%)个菌株;在株水平基因分型上,93(40.8%)株为独立基因型;其余菌株基因型分别包含2～10株结核分枝杆菌,共构成132个基因簇。结论:甘肃省临床分离结核分枝杆菌菌株存在丰富的基因多态性,MLVA方法具有较高的基因分型能力,可以满足结核分枝杆菌株水平DNA分型的需要。甘肃省临床分离结核分枝杆菌菌株主要为北京家族基因型菌株。
OBJECTIVE: To understand the genotypes of Mycobacterium tuberculosis isolates in Gansu Province by multi-site variable number tandem repeat (MLVA) typing. Methods: The 15 VNTR loci were selected to detect the DNA of clinical isolates. The DNA fingerprinting was analyzed by BioNumerics4.5 software and the results of cluster analysis were obtained. Results: 228 strains of Mycobacterium tuberculosis were divided into 4 major gene groups and 7 major genotypes, including 13 (5.7%), 3 (1.3%), 7 (3.1%), 1 (0.4%), 171 75.0%), 31 (13.6%) and 2 (0.9%) strains, respectively. 93 (40.8%) were independent genotypes at strain level. The remaining genotypes included 2 to 10 tuberculosis branches Bacillus, a total of 132 gene clusters. CONCLUSION: There are abundant gene polymorphisms in clinical isolates of Mycobacterium tuberculosis in Gansu Province. The MLVA method has high genotyping ability and can meet the needs of DNA typing of Mycobacterium tuberculosis strains. The clinical isolates of Mycobacterium tuberculosis in Gansu Province are mainly Beijing family genotype strains.