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由尖孢镰刀菌萎蔫专化型[Fusarium oxysporum f.sp.vasinfectum(Atk,)Synder &Hansen]引起的棉花枯萎病在我国及世界许多产棉国家都可造成严重危害。我国棉花枯萎病菌存在明显的生理分化,并具有与其它国家不同的、独特的生理小种。为了深入研究病菌致病性及寄生专化性的分子机制,首先必须建立病菌的遗传转化系统。 Case等 1979年将外源9a-2基因导入粗糙脉孢菌(Neurospora carassa)染色体基因组,开创了丝状真菌转化的先河。近年来,具有显性选择标记的潮霉素(Hygromycin B)等抗药性基因的利用,加快了在工农业生产上具有重要意义的各类真菌转化技术的发展。迄今已有玉米小斑病菌(Cochliobolus heterostrophus)、玉米黑粉病菌(Ustilago maydis)等10余种植物病原真菌建立了原生质体转化系统。本文首次报道携带潮霉素抗性基因hph(潮霉素磷酸转移酶基因)的两种质粒pAN7-1和pDH25转化棉花枯萎病菌的试验结果。
Cotton blight caused by Fusarium oxysporum f.sp.vasinfectum (Atk, Synder & Hansen) can cause serious harm in our country and in many cotton producing countries around the world. There are obvious physiological differentiation of Fusarium oxysporum f. Sp. In China, and have different and unique physiological races. In order to further study the pathogenic and parasitic pathogenic bacteria molecular mechanism, we must first establish a germ genetic transformation system. In 1979, Case et al introduced the exogenous 9a-2 gene into the genome of Neurospora carassa chromosomes, creating a precedent for the transformation of filamentous fungi. In recent years, the use of drug-resistant genes such as hygromycin B with dominant selection markers has accelerated the development of various fungal transformation technologies of great industrial and agricultural production. To date, more than 10 plant pathogenic fungi such as Cochliobolus heterostrophus and Ustilago maydis have established protoplast transformation systems. This paper reports for the first time the results of the transformation of two strains of cotton pY7-1 and pDH25 harboring the hygromycin resistance gene hph (hygromycin phosphotransferase gene) to Fusarium oxysporum f.