目的探讨血清胸苷激酶1(TK1)、糖类抗原199(CA199)、糖类抗原724(CA724)联合检测对结直肠癌诊断的临床应用价值。方法采用电化学发光法对77例结直肠癌患者、61例结直肠良性病变患者和40例健康体检者的血清CA199、CA724含量进行检测,采用免疫印迹-增强化学发光法对各组血清TK1含量进行检测。结果结直肠癌组的TK1、CA199和CA724水平显著高于结直肠良性病变组患者和正常对照组(P均<0.05),结直肠良性病变患者的血清TK1、CA199和CA724水平显著高于正常对照组(P<0.05)。单项检测TK1对结直肠癌诊断的敏感度、特异度和准确度分别为40.3%、75.4%、55.8%,CA199为44.2%、88.5%、63.8%,CA724为45.5%、77.0%、59.4%;联合检测TK1、CA199和CA724为64.9%、68.9%、65.9%。结论 TK1、CA199和CA724水平对于结直肠癌的诊断、鉴别诊断具有重要的临床价值,三者联合检测可提高结直肠癌的检出率。 Objective To investigate the clinical value of combined detection of serum thymidine kinase 1 (TK1), carbohydrate antigen 199 (CA199) and carbohydrate antigen 724 (CA724) in the diagnosis of colorectal cancer. Methods The levels of serum CA199 and CA724 in 77 patients with colorectal cancer, 61 patients with colorectal benign disease and 40 healthy subjects were detected by electrochemiluminescence. The contents of TK1 in serum of each group were detected by immunoblotting-enhanced chemiluminescence Test. Results The levels of TK1, CA199 and CA724 in colorectal cancer group were significantly higher than those in benign colorectal benign disease group and normal control group (all P <0.05). The levels of serum TK1, CA199 and CA724 in colorectal benign lesions were significantly higher than those in normal control Group (P <0.05). The sensitivity, specificity and accuracy of single test for the diagnosis of colorectal cancer were 40.3%, 75.4% and 55.8%, respectively. The levels of CA199 were 44.2%, 88.5% and 63.8%, respectively. The positive rate of CA724 was 45.5%, 77.0% and 59.4% respectively. Joint detection of TK1, CA199 and CA724 was 64.9%, 68.9%, 65.9%. Conclusion The levels of TK1, CA199 and CA724 have important clinical value in the diagnosis and differential diagnosis of colorectal cancer. The combined detection of the three can improve the detection rate of colorectal cancer.