论文部分内容阅读
目的探讨病毒性肝炎患者血清中 IL-12与可溶性肿瘤坏死因子(sTNFR)的改变及其临床意义。方法双抗体夹心酶联免疫吸附法(ELISA)检测64例病毒性肝炎患者及20例正常对照血清 IL-12与sTNFR 水平。结果病毒性肝炎患者血清IL-12与 sFNFR 水平明显高于正常对照,与临床病型关系密切,在慢性肝炎、急性肝炎及重型肝炎依次升高,差异有显著性意义(P<0.01)。恢复期血清 IL-12水平迅速复常(P>0.05);血清 sTNFR 在急性肝炎恢复期,迅速复常(P>0.05),在慢性肝炎恢复期及部分重型肝炎恢复期血清 sTNFR 水平仍高于正常(P<0.05)。血清 IL-12与 sTNFR 水平与血清总胆红素及谷丙转氨酶水平呈正相关。结论检测血清 IL-12与 sTNFR 水平,可判断病情的严重程度及预后,指导临床诊断及治疗。
Objective To investigate the changes of serum IL-12 and soluble tumor necrosis factor (sTNFR) in patients with viral hepatitis and its clinical significance. Methods Serum levels of IL-12 and sTNFR in 64 patients with viral hepatitis and 20 normal controls were detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Results Serum levels of IL-12 and sFNFR in patients with viral hepatitis were significantly higher than those in controls, and were closely related to clinical types. The levels of serum IL-12 and sFNFR in patients with viral hepatitis were significantly higher than those in patients with chronic hepatitis, acute hepatitis and severe hepatitis (P <0.01). Serum levels of sTNFR at recovery convalescence were rapidly normalized (P> 0.05). Serum levels of sTNFR at convalescent stage of chronic hepatitis and convalescent severe hepatitis were still higher than those at recovery stage Normal (P <0.05). Serum IL-12 and sTNFR levels were positively correlated with serum total bilirubin and alanine aminotransferase levels. Conclusion Detection of serum IL-12 and sTNFR levels can determine the severity of the disease and prognosis, to guide the clinical diagnosis and treatment.