右归丸方含药血清对BMSC增殖、骨向分化的干预作用研究

来源 :中华中医药学刊 | 被引量 : 0次 | 上传用户:yu8886882
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的:观察右归丸含药血清对骨髓基质干细胞的骨向分化诱导作用,通过右归丸含药血清对骨髓基质干细胞Akt、mTOR磷酸化水平的影响,探讨右归丸含药血清促骨髓基质干细胞骨向分化的部分作用机制。方法:制备高中低3个剂量的右归丸大鼠含药血清,提取大鼠骨髓基质干细胞,传至第3代,将细胞随机分为5组:空白对照组、空白血清组、低剂量含药血清组、中剂量含药血清组、高剂量含药血清组。分别给予不同浓度血清及PBS干预24 h后,检测各组细胞中碱性磷酸酶活性、骨钙素表达水平及Akt、mTOR磷酸化水平。结果:空白对照组与空白血清组之间比较,ALP、Osteocalcin、p-Akt、p-mTOR表达水平无统计学差异(P>0.05),与空白对照组和空白血清组比较,3个剂量含药血清组细胞中ALP活性及Osteocalcin、p-Akt、p-mTOR表达水平均不同程度提高(P>0.01),但中剂量和高剂量含药血清组ALP活性及Osteocalcin、p-Akt、p-mTOR表达水平均无显著性差异(P>0.05)。结论:右归丸含药血清具有一定的促进骨髓基质干细胞骨向分化的作用,该作用可能是通过提高Akt、mTOR的磷酸化水平而实现的。 OBJECTIVE: To observe the effect of Youguiwan containing serum on bone marrow stromal differentiation induced by bone marrow stromal cells, and to study the effect of Youguiwan containing serum on the phosphorylation of Akt and mTOR in bone marrow stromal cells Stem cells to differentiate part of the mechanism of action. Methods: Three doses of high, medium and low dose of Yougui Wan containing serum were prepared and the rat bone marrow stromal cells were extracted and passaged to the third passage. The cells were randomly divided into five groups: blank control group, blank serum group, low dose drug Serum group, middle dose serum group and high dose serum group. After being treated with different concentrations of serum and PBS for 24 hours, the activity of alkaline phosphatase, osteocalcin, phosphorylation of Akt and mTOR in each group were detected. Results: There was no significant difference in the expression of ALP, Osteocalcin, p-Akt and p-mTOR between blank control group and blank serum group (P> 0.05). Compared with blank control group and blank serum group, The ALP activity and the expression of Osteocalcin, p-Akt and p-mTOR in the serum of the drug-treated group were all increased to some extent (P> 0.01) mTOR expression levels were no significant difference (P> 0.05). Conclusion: Yougui pill containing serum can promote the bone marrow stromal differentiation to a certain extent, and this effect may be achieved by increasing the phosphorylation level of Akt and mTOR.
其他文献
回 回 产卜爹仇贱回——回 日E回。”。回祖 一回“。回干 肉果幻中 N_。NH lP7-ewwe--一”$ MN。W;- __._——————》 砧叫]们羽 制作:陈恬’#陈川个美食 Back to yield
农村党支部和村民委员会作为两个性质和功能相异的农村基层组织,共存于同一社区,发挥着各自的村治功能。在村民自治刚开始实施的时候,农村在“一元化”权力结构影响下,党支部既是
本文简述了反对归化,提倡异化的现代翻译理念,对《飘》的五个译本从词汇学、美学、语法和文化的维度分析了不同方法翻译实践的得失,论证了异化翻译的必要性。
本文从目前开展的中学教师继续教育培训工作的实际出发,对有关问题进行探讨.一、开展教师继续教育培训是学校整体工作的组成部分,"职前职后贯道"是学校两大办学功能一体化的
近日,一家大型网购平台出售378万元“天价”茅台老酒的消息引发网友关注和热议,不少网友在惊叹价格之高的同时,也提出疑问,如此高价的茅台老酒是真的吗?由谁来鉴定真伪?中国收