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目的 探讨肝细胞癌 (HCC)微卫星变异的特点及其与临床病理表现的相关性。方法采用毛细管电泳DNA分析系统 ,对 5 6例HCC中 8号染色体上 10个微卫星的杂合子丢失 (LOH)、微卫星不稳定性 (MSI)和等位基因失衡 (AI) 3种变异特征进行检测。结果 5 6例HCC在 8号染色体上 10个基因位点发生LOH的总频率为 6 6 1% (37/ 5 6 ) ,LOH以D8S2 6 1最高为 5 3 5 % (2 3/ 4 3) ,其次为D8S172 1(5 2 5 % )和D8S1771(5 2 5 % )。D8S2 77基因位点 ,血清HBsAg阳性患者的LOH频率显著高于HBsAg阴性者 (P <0 0 1) ,D8S2 6 1、D8S2 98和D8S1733基因位点 ,血清HBsAg阴性患者的LOH频率显著高于HBsAg阳性者 (P <0 0 1) ;D8S2 98和D8S1771基因位点 ,直径 >3cm肿瘤的LOH率明显高于≤ 3cm组 (P <0 0 5和P <0 0 1) ;在D8S172 1基因位点 ,无包膜或包膜不完整的肿瘤的LOH显著高于包膜完整的肿瘤 (P <0 0 1) ;D8S2 98和D8S1771基因位点 ,肝内转移者的LOH明显高于无肝内转移者 (P <0 0 5 )。MSI的频率为 12 5 % (7/ 5 6 ) ,AI的频率为 19 6 % (11/ 5 6 )。结论HCC在 8号染色体上存在广泛的微卫星变异 ,其中LOH方式在HCC的发生和发展过程中起重要作用 ,MSI的作用次之。特定基因位点的LOH与临床和病理学参数有一定的相关性
Objective To investigate the characteristics of microsatellite mutation in hepatocellular carcinoma (HCC) and its correlation with clinicopathological features. The method of capillary electrophoresis DNA analysis system, the 56 heterozygous patients with HCC 10 chromosome 8 microsatellite loss (LOH), 3 intraspecific variation wherein microsatellite instability (MSI) and allelic imbalance (AI) Test. Results The total frequency of LOH in 106 loci on chromosome 8 was 6 6 1% (37/5 6), and the highest LOH in D8S2 6 1 was 5 3 5% (2 3/4 3) , Followed by D8S172 1 (52.5%) and D8S1771 (52.5%). D8S2 77 loci, LOH frequency of positive patient serum HBsAg significantly higher HBsAg negative (P <0 0 1), D8S2 6 1, D8S2 98 and D8S1733 locus, LOH frequency HBsAg negative patient sera significantly higher HBsAg positive (P <0 0 1); D8S2 98 and D8S1771 loci, diameter> 3cm LOH significantly higher than the rate of tumor ≤ 3cm group (P <0 0 5 and P <0 0 1); in the locus D8S172 1 The LOH of point-null, non-envelopment or incomplete envelopment was significantly higher than that of intact envelopment (P <0.01). The LOH of D8S2 98 and D8S1771 locus and intrahepatic metastasis was significantly higher than that of non-hepatic Transfer (P <0 0 5). The frequency of MSI was 12 5% (7/56) and that of AI was 19 6% (11/56). Conclusion HCC has a wide range of microsatellite mutations on chromosome 8, of which LOH plays an important role in the development and progression of HCC, followed by MSI. LOH at a particular locus has some correlation with clinical and pathological parameters