[Objective] To study the extraction, qualitative identification and quantitative assay of polysaccharides from the leaves of Prunus pseudocerasus Lindl. (PPP), so as to provide reference for its comprehensive application. [Method] Polysaccharides were extracted with the method of water exaction and alcohol precipitation after the leaves were defatted with 95% EtOH and petroleum ether. Then, the protein in PPP was removed by Sevag method. A series of experiments were used to identify the PPP, including Molish test (α-naphthol test), anthrone-sulfuric acid method test, fehling test, fourier transform infrared spectrometry (FT-IR), and iodine test. The content of PPP was determined by anthrone-sulphuric acid colrimetry method. [Result] P. pseudocerasus polysaccharides were grey white in color. The content of polysaccharides in P. pseudocerasus leaves was 29.7 mg/g. [Conclusion] The method is simple, sensitive, reproducible, and reliable. [Objective] To study the extraction, qualitative identification and quantitative assay of polysaccharides from the leaves of Prunus pseudocerasus Lindl. (PPP), so as to provide reference for its comprehensive application. [Method] Polysaccharides were extracted with the method of water exaction and Then, the protein in PPP was removed by Sevag method. A series of experiments were used to identify the PPP, including Molish test (α-naphthol test), anthrone- sulfuric acid method test, fehling test, fourier transform infrared spectrometry (FT-IR), and iodine test. The content of PPP was determined by anthrone-sulphuric acid colrimetry method. [Result] P. pseudocerasus polysaccharides were gray in color. content of polysaccharides in P. pseudocerasus leaves was 29.7 mg / g. [Conclusion] The method is simple, sensitive, reproducible, and reliable.