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目的:研究生长抑素(somatostatin,SST)与大鼠肝星状细胞(hepaticstellatecell,HSC)增生、凋亡的关系.方法:分离、培养的原代大鼠HSC,经不同浓度SST(10-6-10-10mol/L)处理72h后,以吖啶橙(acridineorange,AO)/溴乙啶(ethidiumbromide,EB)荧光染色法、末端核苷酸转移酶介导的脱氧三磷酸尿苷原位缺口末端标记法(terminaldeoxynucleotidyltransferase-mediateddUTPnickendlabeling,TUNEL)、透射电镜及流式细胞术检测其凋亡率的改变.SST作用120h后,以甲基噻唑基四唑(methylthiazolyltetrazolium,MTT)比色法检测HSC增生率的变化.并通过对细胞动力学的观察,探讨SST的作用机制.结果:SST可通过剂量依赖方式抑制HSC增生,提高HSC凋亡率.浓度为10-6mol/L或10-7mol/L时效果尤为显著.G0/G1期阻滞是SST发挥作用的重要途径.结论:低剂量SST即可抑制HSC增生,并促进其凋亡,提示SST在抗肝纤维化方面具有潜在价值.
OBJECTIVE: To study the relationship between somatostatin (SST) and proliferation and apoptosis of rat hepatic stellate cells (HSC) .Methods: Primary rat HSCs isolated and cultured were cultured in different concentrations of SST (10-6 After treated with 10-10mol / L for 72h, the cells were stained with acridine orange (AO) / ethidiumbromide (EB) fluorescent staining, terminal nucleotide transferase mediated deoxyriphosphate triphosphate in situ nick TUNEL, transmission electron microscopy and flow cytometry were used to detect the apoptotic rate.After 120 hours of SST, MTT colorimetric assay was used to detect the rate of HSC hyperplasia .Through the observation of cell kinetics, the mechanism of SST was explored.Results: SST could inhibit the proliferation of HSC in a dose-dependent manner and increase the apoptosis rate of HSC.When concentration was 10-6mol / L or 10-7mol / L Especially in G0 / G1 phase.Conclusion: Low dose SST can inhibit HSC proliferation and promote its apoptosis, suggesting that SST has potential value in anti-hepatic fibrosis.