自细胞融合技术问世以来，已被广泛用于研究肿瘤细胞的恶性表型的表达与调控。这一技术的关键步骤是建立一个次黄嘌呤鸟嘌呤磷酸核糖转移酶（ＨＧＰＲＴ）缺陷型并保持亲代特征的细胞系，这样才可以用ＨＡＴ培养液选择杂交瘤细胞。本文采用浓度梯度递增法从小鼠肝癌低淋巴道转移细胞ＨＣａ－Ｐ中选出８－氮鸟嘌呤（８－Ａｇ）抗性细胞，该细胞既对ＨＡＴ敏感又保持低淋巴道转移特征。 Since the advent of cell fusion technology, it has been widely used to study the expression and regulation of the malignant phenotype of tumor cells. The key step in this technique is to establish a hypoxanthine guanine phosphoribosyl transferase (HGPRT)-deficient and maintain parental cell line so that hybridoma cells can be selected using HAT medium. In this study, 8-aminoguanine (8-Ag)-resistant cells were selected from HCa-P cells in mice with low-lying lymph node metastases using a gradient-increment method. The cells were both sensitive to HAT and maintained low lymphatic metastasis characteristics.