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本文通过建立ＨＲＰ逆行追踪结合抗ＦＯＳ和抗酪氢酸羟化酶（ＴＨ）的免疫组织化学三重标记技术，在延髓见到以下七种标记细胞：ＦＯＳ，ＨＲＰ，ＴＨ单标细胞，ＦＯＳ／ＨＲＰ，ＦＯＳ／ＴＨ，ＨＲＰ／ＴＨ双标细胞，ＦＯＳ／ＨＲＰ／ＴＨ三标细胞。这些细胞主要分布于延髓中、尾段由孤束核、腹外侧区以及在两者之间的网状结构共同组成的弧形带状区──“延髓内脏带”。本文结果表明大鼠“延髓内脏带”向杏仁核直接投射的儿茶酚胺能神经元中约有４８％对内脏伤害性刺激起反应，提示“延髓内脏带”及其向杏仁核的儿茶酚胺能投射通路参与机体应激反应的调控过程。
In this study, the following seven labeled cells were observed in the medulla by HRP retrograde tracing combined with anti-FOS and anti-tyrosine hydroxylase (TH) immunohistochemical triple labeling: FOS, HRP, TH single cell, FOS / HRP , FOS / TH, HRP / TH double-labeled cells, FOS / HRP / TH triple-labeled cells. These cells are mainly located in the medulla oblongata, the caudal segment consists of arcuate zonal zones consisting of the nucleus tractus solitarius, the ventrolateral region and the network between them - the “medullary visceral zone.” Our results show that about 48% of catecholaminergic neurons directly projected from the “medullary visceral zone” to the amygdala respond to visceral noxious stimulation, suggesting that the “medullary visceral zone” and its involvement in the amygdala catecholaminergic pathways Body stress response regulation process.