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目的 :用RAPD技术检测人肺巨细胞癌高低转移细胞株的遗传改变。方法 :人肺巨细胞癌细胞系高转移细胞株PLA 80 1D和低转移株PLA 80 1C ,用 10 0条各 10个碱基的随机引物经PCR扩增 ,扩增产物在含溴化乙锭的 1 5 %琼脂糖凝胶中电泳 ,紫外透射仪上观察照相。结果 :10 0条引物绝大多数能扩增出明显的条带 ,多数引物产生的条带在高低转移株之间无差异 ,表现为单态性 ;其中 17条引物产生多态性 ,表现为带的有无、移动和强弱差异。结论 :用RAPD技术检测出人肺巨细胞癌高低转移细胞株基因组DNA之间存在差异 ,可能与肿瘤转移表型相关。
Objective: To detect the genetic alteration of human lung cell carcinoma cell line with RAPD by using RAPD technique. METHODS: Human lung giant cell carcinoma cell line, high metastatic cell line PLA 80 1D and low metastatic line PLA 80 1C, were amplified by PCR using 10 10 random primers with 10 bases each. Of 1 5% agarose gel electrophoresis, UV transmission instrument observed photography. Results: Most of the 10 primers could amplify the obvious bands, and most of the bands produced by the primers showed no difference between the high and low metastatic strains, showing monomorphism. Among them, 17 primers produced polymorphisms With or without the band, movement and strength differences. Conclusion: The difference of genomic DNA between human lung cancer cell line and giant cell lung cancer cell line detected by RAPD technique may be related to tumor metastasis phenotype.