目的　本实验为检测肝癌组织中是否有端粒酶活性，端粒酶活性与肝癌临床检测指标ＡＦＰ及高低转移潜能是否相关而设计。　方法　本实验用ＴＲＡＰ法从组织中抽提端粒酶，经反转录并ＰＣＲ 扩增后用地高辛标记的探针作斑点印迹杂交（ 肝癌标本部分） 和ＰＡＧ电泳加银染法显色（ 人肝癌高低转移裸鼠模型） 。　结果　本实验检测了１１ 例肝癌患者有肝癌组织和癌周组织的端粒酶活性，结果肝癌组织中端粒酶活性为９３－８８（单位：ＩＯＤ值） ，癌周组织端粒酶活性为２４－０９，两者的差别有统计学意义（Ｐ＜ ０－００１） 。正常组织（ｎ ＝ ５） 中未检测到端粒酶活性。端粒酶活性与ＡＦＰ浓度的相关系数ｒ＝０ ．７２，与肿瘤大小的相关系数ｒ＝ －０－２９８。人肝癌高转移模型裸鼠ＬＣＩＤ２０ 的端粒酶活性为（７７－６７±６－９３），低转移模型裸鼠ＬＣＩＤ３５ 的为８２－１６ ±１５－９３），两者之间并无显著性差异（ Ｐ＞ ０－０５） 。　结论　肝癌组织的端粒酶活性明显地高于癌周组织，正常组织中未检测到端粒酶活性。端粒酶活性与ＡＦＰ及肿瘤大小无关。端粒酶活性和肝癌的转移潜能可能不直接相关 Objectives This experiment was designed to detect whether there is telomerase activity in liver cancer tissue, and whether telomerase activity is related to the clinical detection index AFP and the high or low metastatic potential of liver cancer. Methods In this experiment, telomerase was extracted from tissues by TRAP method. After reverse transcription and PCR amplification, digoxigenin-labeled probes were used for dot blot hybridization (parts of liver cancer specimens) and PAG electrophoresis plus silver staining ( Human liver cancer metastatic nude mouse model). Results The telomerase activity of liver cancer tissue and pericancerous tissue was detected in 11 patients with hepatocellular carcinoma. The telomerase activity in hepatocellular carcinoma tissue was 93-88 (unit: IOD value), and the telomerase activity in pericancerous tissue was 24 -09, the difference between the two was statistically significant (P < 0-001). No telomerase activity was detected in normal tissues (n = 5). The correlation coefficient between telomerase activity and AFP concentration was r=0. 72, Correlation coefficient with tumor size r = -0-298. The telomerase activity of LCID20 in human high-metastatic model of hepatocellular carcinoma was (77-67±6-93), and that of LCID35 in the low-metastatic model was 82-16±15-93. No significant difference (P> 0-05). Conclusion The telomerase activity of hepatocellular carcinoma is significantly higher than that of the pericancerous tissue. No telomerase activity is detected in normal tissues. Telomerase activity was not associated with AFP and tumor size. Telomerase activity may not be directly related to the metastatic potential of liver cancer