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目的:探讨五味子醇提部位对反复脑缺血再灌注模型小鼠脑能量代谢的影响。方法:将动物随机分成6组,分别灌服尼莫地平片混悬液、银杏叶混悬液及大、小剂量五味子醇提部位混悬液,假手术组(空白组)和模型组给予同体积生理盐水,每日1次,连续给药15d,采用双侧颈总动脉夹闭,建立反复脑缺血再灌注致学习记忆障碍小鼠模型。术后48h后,断头取脑,取一半脑称质量,加9倍生理盐水制成10%脑匀浆,测乳酸含量,乳酸脱氢酶、乙酰胆碱酯酶和ATP酶活力。结果:与模型组比,小剂量五味子醇提部位可明显降低模型小鼠乳酸含量,大剂量五味子醇提部位可显著提高模型小鼠乳酸脱氢酶活力;大、小剂量五味子醇提部位组可显著提高模型小鼠乙酰胆碱酯酶活力,大剂量五味子醇提部位组能显著提高模型小鼠Na+-K+-ATP、Ca2+-ATP酶活力。结论:五味子醇提部位可显著改善反复脑缺血再灌注模型小鼠脑能量代谢。
Objective: To investigate the effect of alcohol extract of Schisandra on cerebral energy metabolism in mice with repeated cerebral ischemia-reperfusion. Methods: The animals were randomly divided into 6 groups: nimodipine suspension, suspension of ginkgo biloba and suspension of large and small doses of Schisandra chinensis, respectively. The sham operation group (blank group) and model group Volume normal saline once a day for 15 days. The bilateral common carotid arteries were used to establish a mouse model of learning and memory impairment caused by repeated cerebral ischemia and reperfusion. After 48h, the brain was decapitated and half of the brain was weighed. The brain homogenate was prepared by adding 9 times of normal saline to measure the content of lactic acid, lactate dehydrogenase, acetylcholinesterase and ATPase activity. Results: Compared with the model group, the small dose of Schisandra alcohol extract can significantly reduce the model mice lactic acid content, high-dose Schisandra alcohol extract can significantly enhance the model mice lactate dehydrogenase activity; large and small doses of Schisandra alcohol extract site group Significantly increased the acetylcholinesterase activity in model mice. The high dose of Schisandra alcohol extract could significantly increase the Na + -K + -ATP and Ca2 + -ATPase activities in model mice. Conclusion: Fructus schisandrae extract can significantly improve brain energy metabolism in mice with repeated cerebral ischemia-reperfusion.