论文部分内容阅读
目的探讨前列腺生长因子（ＰｒＧＦ）在良性前列腺增生症（ＢＰＨ）发生中的作用。方法同位素３Ｈ－ＴｄＲ掺入法测定人正常及ＢＰＨ前列腺组织匀浆和提纯物中ＰｒＧＦ促传代培养的人包皮成纤维细胞增殖的活性。结果ＢＰＨ组匀浆和提纯物中的ＰｒＧＦ促包皮成纤维细胞增殖活性均高于正常前列腺组（Ｐ＜０．００５）；ＢＰＨ组和正常组的提纯物在达到一定浓度时显示增殖抑制，表明有ＰｒＧＦ的抑制成分存在；ＢＰＨ组显示增殖抑制的蛋白浓度高于正常组。结论ＰｒＧＦ活性升高及与其抑制成分的平衡紊乱，对ＢＰＨ的发生有重要意义
Objective To investigate the role of prostatic growth factor (PrGF) in the pathogenesis of benign prostatic hyperplasia (BPH). Methods The isotope 3H-TdR incorporation method was used to determine the proliferation of human foreskin fibroblasts in human normal and BPH prostate tissue homogenates and purified PrGF. Results The proliferation of PrGF-stimulated fibroblasts in both BPH group and purified group were significantly higher than those in normal prostate group (P <0.005). The purified BPH group and normal group showed inhibition of proliferation at a certain concentration Inhibition of PrGF components exist; BPH group showed that the proliferation inhibition of protein concentration was higher than the normal group. Conclusions PrGF activity and the balance of its inhibitory components are disturbed, which is of great significance for the occurrence of BPH