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目的：探讨槲皮素对实验性乳腺癌中的肿瘤血管生成抑制作用。方法：本实验建立了DMBA诱导的乳腺癌动物模型。79只雌性SD大鼠随机分为四组：A组（DMBA），B组（DMBA＋TAM），C组（DMBA＋槲皮素），D组（空白对照）。喂养持续28周后，进行光镜观察、MVD计数，抗bFGF、VEGF及H－ras的免疫组化分析。结果：1）A组大鼠乳腺肿瘤发生率及癌灶平均直径（75．2％、2．37cm）明显高于B组（40．9％、1．82cm），C组（45．5％、1．71cm）及D组（0％、0cm），其差异均有显著意义（P＜0．05），B组和C组差异无显著意义（P＞0．05）；2）MVD、VEGF、bFGF及H－ras免疫组化分析显示：A组与B组和C组差异有显著意义（P＜0．05），B组和C组无显著性差异（P＞0．05）。结论：槲皮素有降低DMBA诱导的SD大鼠乳腺癌发生率及抑制其生长的作用，其机制可能与以下因素有关：1）抑制ras基因活性，从而抑制肿瘤细胞的增殖及血管生成；2）抑制VEGF，bFGF等血管生长因子的活性，肿瘤血管生成过程受阻，致乳腺癌组织内MVD降低。
Objective: To investigate the inhibitory effect of quercetin on tumor angiogenesis in experimental breast cancer. Methods: This experiment established a DMBA-induced breast cancer animal model. 79 female SD rats were randomly divided into four groups: group A (DMBA), group B (DMBA+TAM), group C (DMBA+quercetin), and group D (blank control). After 28 weeks of feeding, light microscopy, MVD counts, immunohistochemical analysis of anti-bFGF, VEGF, and H-ras were performed. Results: 1) The incidence of breast tumors and the average diameter of the foci in group A (75.2%, 2.37cm) were significantly higher than those in group B (40.9%, 1.82cm), and in group C (45.5%). (1, 7cm) and D group (0%, 0cm), the differences were significant (P <0.05), there was no significant difference between group B and C (P> 0.05); 2) MVD, Immunohistochemical analysis of VEGF, bFGF, and H-ras showed that the difference between group A and group B and group C was significant (P<0.05), and there was no significant difference between group B and group C (P>0.05). Conclusion: Quercetin can reduce the incidence of DMBA-induced breast cancer in SD rats and inhibit its growth. Its mechanism may be related to the following factors: 1) It inhibits the activity of ras gene and inhibits the proliferation and angiogenesis of tumor cells.2 ) Inhibition of VEGF, bFGF and other angiogenic growth factor activity, tumor angiogenesis blocked, resulting in reduced MVD in breast cancer tissue.