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目的:从中药厚朴中分离得到群体感应抑制剂,并对其活性进行评价。方法:利用铜绿假单胞菌QSIS-las I对中药厚朴的粗提物进行活性检测及追踪,采用生物自显影薄层色谱、制备薄层色谱和高效液相色谱技术,分离纯化厚朴中的活性化合物,应用核磁共振波谱解析确定其结构。分别利用分光光度法及地衣酚法测定绿脓菌素和鼠李糖脂的产量,并通过半固体培养基检测铜绿假单胞菌的swarming运动,利用RT-PCR检测与QS调控相关基因的m RNA表达的影响。结果:厚朴粗提物具有群体感应抑制活性,其活性化合物结构鉴定为和厚朴酚。亚抑菌浓度下的和厚朴酚能明显抑制毒力因子如鼠李糖脂和绿脓菌素的产量以及swarming运动,且能够有效抑制QS相关基因m RNA水平的表达(P<0.05)。结论:和厚朴酚是一种新的群体感应抑制剂,可能发展成为一种治疗细菌感染的潜在新药。
OBJECTIVE: To isolate mass induction inhibitors from Magnolia officinalis and evaluate its activity. Methods: Pseudomonas aeruginosa QSIS-las I crude extract of Magnolia officinalis activity testing and tracking, using autoradiographic thin-layer chromatography, preparative thin-layer chromatography and high performance liquid chromatography, separation and purification of Magnolia officinalis Of the active compound, the application of nuclear magnetic resonance spectroscopy to determine its structure. The yields of pyocyanin and rhamnolipid were determined by spectrophotometry and the method of lichen phenol respectively. The swarming motility of Pseudomonas aeruginosa was detected by semi-solid medium. The mRNA and protein expression of m Effect of RNA expression. Results: Magnolia officinalis crude extracts had a population-based inhibitory activity and its active compound structure was identified as honokiol. Honokiol could inhibit the production of virulence factors such as rhamnolipid and pyocyanin, and swarming motility significantly, and could effectively inhibit the expression of m RNA level of QS related genes (P <0.05). Conclusions: Honokiol is a new group-sensing inhibitor that may develop into a potentially new drug for the treatment of bacterial infections.