Objective To study the immune regulative function of Sertoli cell on testis local infection Methods Ureaplasma urealyticum (UU) was directly injected into bladders of FasL transgenic mice and wild-type mice, which mimicked an ascending infectious way. At week 1, 2 and 3 after injection respectively, the mice were killed to observe the pathological alterations in testis section. And at the same time cytokines was tested by immunohistochemistry. Comparison of levels of FasL, TGF-β, IL-1α, and IL-6 between UU-infected and control groups of wild mice and FasL transgenic mice was made respectively. Then the capability of Sertoli cell (FasL+) to mediate apoptosis of Fas+ cells between wild control and wild UU-infected groups was analyzed. Results The pathological changes of testis in FasL transgenic mice were more seriously compared with wild counterpart and the changing mode of cytokines secreted by Sertoli cells were different between the two kinds of mice. The UU-infected Sertoli cells increased Fas+ Jurkat cell apoptosis. Conclusions High expression of FasL in FasL transgenic mice can influence the cytokines secretion during anti-infection, thus affecting the testis immune response to infection and immune balance. The high expression of FasL is not beneficial for body’s anti-inflection immune response. Objective To study the immune regulative function of Sertoli cell on testis local infection Methods Ureaplasma urealyticum (UU) was directly injected into bladders of FasL transgenic mice and wild-type mice, which mimicked an ascending of infectious way. At week 1, 2 and 3 after And the same time cytokines was tested by immunohistochemistry. Comparison of levels of FasL, TGF-β, IL-1α, and IL-6 between UU-infected and control the groups of wild mice and FasL transgenic mice were made respectively. Then the capability of Sertoli cell (FasL +) to mediate apoptosis of Fas + cells between wild control and wild UU-infected groups was analyzed. Results The pathological changes of testis in FasL transgenic mice were more seriously compared with the wild counterpart and the changing mode of cytokines secreted by Sertoli cells were different between the two kinds of mice. The UU-infected Sertoli cells Increased Fas + Jurkat cell apoptosis. Conclusions High expression of FasL in FasL transgenic mice can influence the cytokines secretion during anti-infection, thus affecting the testis immune response to infection and immune balance. The high expression of FasL is not beneficial for body’s anti-inflection immune response.