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目的:检测食管鳞状细胞癌组织中RECK蛋白的表达及微血管密度(MVD)。方法:应用免疫组织化学SP法检测62例食管鳞状细胞癌组织、31例癌旁不典型增生组织及62例正常食管黏膜组织中RECK蛋白的表达与MVD。结果:食管鳞状细胞癌组织、癌旁不典型增生组织及正常黏膜组织中RECK蛋白的阳性表达率依次增高,分别为59.7%(37/62)、71.0%(22/31)、85.5%(53/62),3组间比较差异有统计学意义(P<0.05);RECK蛋白的表达与食管鳞状细胞癌的组织学分级、浸润深度及淋巴结转移密切相关(P<0.05)。MVD与食管鳞状细胞癌的浸润深度及淋巴结转移密切相关(P<0.05)。结论:RECK和MVD在食管鳞状细胞癌的黏膜上皮癌变及浸润、转移中起重要作用,2者联合检测可望成为食管鳞状细胞癌早期诊断和预后判断的分子指标之一。
Objective: To detect the expression of RECK protein and microvessel density (MVD) in esophageal squamous cell carcinoma. Methods: Immunohistochemical SP method was used to detect the expression of RECK protein and MVD in 62 cases of esophageal squamous cell carcinoma, 31 cases of adjacent atypical hyperplasia and 62 cases of normal esophageal mucosa. Results: The positive rates of RECK protein in esophageal squamous cell carcinoma, adjacent atypical hyperplasia and normal mucosa were 59.7% (37/62), 71.0% (22/31) and 85.5% 53/62). There was significant difference between the three groups (P <0.05). The expression of RECK protein was closely related to histological grade, depth of invasion and lymph node metastasis in esophageal squamous cell carcinoma (P <0.05). MVD and esophageal squamous cell carcinoma invasion depth and lymph node metastasis are closely related (P <0.05). Conclusions: RECK and MVD play an important role in the carcinogenesis, invasion and metastasis of esophageal squamous cell carcinoma. The combined detection of RECK and MVD may be one of the molecular indicators for the early diagnosis and prognosis of esophageal squamous cell carcinoma.