论文部分内容阅读
为了阐明Cr6+污染对延胡索的毒害机理,探索重金属对延胡索生长发育的影响。采用土培试验,研究不同浓度的Cr6+(0、50、75、100、150、200、250、300 mg/kg)对延胡索生长、抗氧化酶活性和Cr6+积累等的影响。结果表明,延胡索在Cr6+污染下生长受抑制,生物量下降;Cr6+污染能明显影响延胡索抗氧化酶活性,即超氧化物歧化酶(SOD)活性随Cr6+浓度的升高而逐渐降低,过氧化物酶(POD)的活性随Cr6+浓度的升高而逐渐增加,过氧化氢酶(CAT)活性均随Cr6+浓度的升高而先增后减;Cr6+污染能明显影响延胡索叶绿素含量、可溶性糖含量、丙二醛(MDA)含量等。叶绿素含量与可溶性糖的含量随着Cr6+浓度的升高而先增后减,而MDA含量逐渐增加。随着Cr6+污染浓度的升高,延胡索对Cr6+的积累在地下块茎逐渐增多,在Cr6+浓度为150 mg/kg时Cr6+含量增加速度最大,生产上应该注意土壤中的Cr6+含量。延胡索栽培中应尽量选择Cr6+污染较少的土地进行。
In order to elucidate the toxic mechanism of Cr6 + pollution on Corydalis yanhusuo and explore the effect of heavy metals on the growth and development of Corydalis yanhusuo. The effects of Cr6 + (0, 50, 75, 100, 150, 200, 250 and 300 mg / kg) on the growth, antioxidant enzyme activity and Cr6 + accumulation of Corydalis yanhusuo were studied by soil culture. The results showed that the growth of corydalis was inhibited under Cr6 + pollution and the biomass was decreased. Cr6 + pollution could significantly affect the activity of antioxidant enzyme of Corydalis yanhusuo, that is, the activity of superoxide dismutase (SOD) decreased gradually with the increase of Cr6 + concentration. The activity of POD increased with the increase of Cr6 + concentration. CAT activity increased first and then decreased with the increase of Cr6 + concentration. Cr6 + pollution could significantly affect chlorophyll content, soluble sugar content, Malondialdehyde (MDA) content and so on. Chlorophyll content and soluble sugar content increased first and then decreased with the increase of Cr6 + concentration, while MDA content increased gradually. With the increase of Cr6 + concentration, the accumulation of Cr6 + in the underground tubers of Corydalis increased gradually. When Cr6 + concentration was 150 mg / kg, the Cr6 + content increased most rapidly and the production should pay attention to the content of Cr6 + in the soil. Corydalis cultivation should try to choose Cr6 + less contaminated land.