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目的通过生物信息学方法了解小麦过敏原Tri a Bd 27k的结构特征,为小麦变态反应性疾病的诊断和预防提供依据。方法在Uniprot数据库中获得Tri aBd 27k蛋白序列,通过DNAStar预测B细胞抗原表位,NetMHCⅡ和Syfpeithi预测T细胞抗原表位;使用Swiss-Model软件预测其三维结构并用Ramachandran进行稳定性评估。在Uniport数据库搜索Tri a Bd 27k的同源蛋白,应用Clustalx1.83及Mega3.1构建同源进化树。结果小麦过敏原Tri a Bd 27k的B/T细胞共同抗原表位优势区域为34-42、102-117,Ramachandran软件预测结果显示小麦Tri a Bd 27k蛋白的空间构象稳定。结论通过对小麦过敏原Tri a Bd 27k进行生物信息学分析获得了该过敏原优势区域及三维结构模型,为进一步了解和掌握小麦过敏原Tri a Bd 27k的结构功能打下理论基础。
Objective To understand the structural characteristics of wheat allergen Tri a Bd 27k by bioinformatics methods and provide evidence for the diagnosis and prevention of wheat allergy. Methods The Tri aBd 27k protein sequence was obtained from the Uniprot database. The B cell antigen epitopes were predicted by DNAStar. NetMHC Ⅱ and Syfpeithi were used to predict T cell epitopes. The three-dimensional structure was predicted by Swiss-Model software and the stability was evaluated by Ramachandran. A homologous protein of Tri a Bd 27k was searched in the Uniport database. Clustalx 1.83 and Mega3.1 were used to construct homologous phylogenetic tree. Results The dominant region of B / T cell common epitopes of Tria Bd 27k was 34-42,102-117. The results of Ramachandran software showed that the spatial conformation of Tribaden 27k was stable. Conclusion The allelopathic predominant region and the three-dimensional structural model were obtained by bioinformatics analysis of the wheat allergen Tri a Bd 27k, laying a theoretical foundation for further understanding and mastering the structural function of wheat allergen Tri a Bd 27k.