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目的:探讨急性淋巴细胞白血病(ALL)微小残留病的检测方法,并进一步研究了该方法对完善微小残留病检测的价值和临床意义.方法:采用PCR结合限制性内切酶酶切、异源双链形成分析的方法,分析了30例急性淋巴细胞白血病患者发病及病程中的TCR_γ、IgH基因重排.结果:30例ALL患者, 16例呈TCR_γ,重排,6例呈IgH重排,可作为微小残留病的标志.10例进行了酶谱分析和异源双链形成实验的患者,每例均有不同的V区参与重排,6例形成互不相同的异源双链.10例重排中5例有新的N区插入,形成了新的酶切位点与异源双链,亦即产生新的基因标志.结论:PCR结合限制性内切酶酶谱和异源双链形成的方法,能进一步区别不同的克隆,识别微小残留病.
Objective: To explore the detection method of minimal residual disease in acute lymphoblastic leukemia (ALL) and further study the value and clinical significance of this method for the detection of minimal residual disease. Methods: PCR and restriction enzyme digestion, heterologous The method of double strand formation analysis was used to analyze the TCR_γ and IgH gene rearrangements in the pathogenesis and course of 30 patients with acute lymphoblastic leukemia. Results: 30 patients with ALL, 16 patients presented with TCR_γ, rearrangement, and 6 patients showed IgH rearrangement. Can be used as a marker of minimal residual disease in 10 patients who performed zymographic analysis and heteroduplex formation experiments, each case had a different V region to participate in the rearrangement, 6 cases formed mutually different heterologous double strands. In the case of rearrangement, 5 cases were newly inserted in the N region and a new restriction enzyme site and heteroduplex were formed, ie a new gene marker was generated. Conclusion: PCR combined with restriction endonuclease zymography and heterologous double Chain formation method can further distinguish different clones and identify minimal residual disease.