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本研究分析了微胶囊饲料S1和S2对马氏珠母贝的生长性状与矿化基因nacrein与pif177表达量的影响。实验共设置了5个组合(EG1,EG2,EG3,EG4和CG),其中EG1和EG2分别单投喂饵料S1和S2,EG3和EG4分别投喂S1+亚心形扁藻与S2+亚心形扁藻,CG投喂扁藻。经过60 d的养殖后,比较了各组的生长率及nacrein与pif177的相对表达量的差异。结果表明,各组间的壳宽、壳高绝对增长率与相对增长率均存在显著性差异(p<0.05),EG4具有最大的壳宽、壳高绝对与相对增长率,单喂饲料的EG1和EG2的壳宽与壳高增长率均小于CG或投喂混合饲料的EG3和EG4。各组间外套膜nacrein相对表达量存在显著性差异(p<0.05);EG4中央膜nacrein相对表达量显著高于EG1、EG2和EG3(p<0.05);EG4边缘膜的nacrein相对表达量显著高于EG1和EG3(p<0.05)。各组间外套膜pif177相对表达量存在显著性差异(p<0.05),EG4中央膜和边缘膜的pif177相对表达量均显著高于EG1和EG3组(p<0.05)。壳宽与壳高的增长率与矿化基因的表达量存在正相关性。本研究结果表明研发的胶囊饲料能替代部分单胞藻饵料,为进一步研发珍珠贝人工饲料及开展工厂化养殖提高了参考依据。
In this study, the effects of S1 and S2 microcapsules on growth traits and expression of mineralized genes nacrein and pif177 were studied. Five combinations (EG1, EG2, EG3, EG4 and CG) were set up in the experiment, in which EG1 and EG2 were fed with S1 and S2, respectively, and EG3 and EG4 were fed with S1 + Algae, CG fed algae. After 60 days of culture, the growth rate of each group and the relative expression of nacrein and pif177 were compared. The results showed that there was a significant difference (p <0.05) between the shell width, shell height absolute growth rate and relative growth rate (p <0.05). EG4 had the largest shell width, shell height absolute and relative growth rate, EG1 The growth rates of shell width and shell height of EG2 were lower than those of CG or EG3 and EG4. The expression of nacrein in EG4 was significantly higher than that in EG1, EG2 and EG3 (p <0.05), and the relative expression of nacrein in EG4 marginal membrane was significantly higher At EG1 and EG3 (p <0.05). The relative expression of pif177 in each group was significantly different (p <0.05). The relative expression of pif177 in the central and peripheral membranes of EG4 was significantly higher than that of EG1 and EG3 (p <0.05). There is a positive correlation between the growth rate of shell width and shell height and the expression level of mineralized genes. The results of this study show that the developed capsule feed can replace part of the single-celled bait for reference to further develop the pearl oyster feed and conduct industrialized breeding.