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目的:构建系统表达S100A16基因型小鼠,为研究S100A16基因的生物学功能提供模型动物。方法 :将S100A16cDNA插入CMV启动子下游,构建转基因表达载体,通过显微注射方法建立转基因小鼠。PCR鉴定转基因小鼠的基因型,采用定量PCR(QPCR)方法筛选高表达品系。结果:成功构建S100A16转基因载体,建立了S100A16转基因小鼠,通过PCR及QPCR方法筛选出两个高表达品系(line A,line B)。结论:建立了系统表达S100A16的转基因小鼠,转入的S100A16基因在脂肪、肝脏、肌肉、肺等组织高表达,为研究S100A16的生物学功能特别是在肥胖中的作用及机制提供了动物模型。
OBJECTIVE: To construct a mouse model of S100A16 gene expression and to provide a model animal for studying the biological function of S100A16 gene. Methods: The S100A16 cDNA was inserted into the downstream of CMV promoter to construct transgenic expression vector. The transgenic mice were established by microinjection method. PCR was used to identify the genotype of transgenic mice and the high-expression strains were screened by quantitative PCR (QPCR). Results: S100A16 transgenic vector was successfully constructed and S100A16 transgenic mice were established. Two high expression lines (line A, line B) were screened by PCR and QPCR. CONCLUSIONS: S100A16 transgenic mice were established and the S100A16 gene was highly expressed in adipose, liver, muscle and lung tissues, which provided an animal model for studying the biological functions of S100A16, especially in obesity .