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目的:观察男性不育症患者精浆中α1微球蛋白(α1-MG)和免疫球蛋白G(IgG)含量,及其与精液部分功能指标之间的相互关系。方法:对88例男性不育症患者和22例正常生育者进行精液常规参数测定,根据精子密度将不育症患者分为精子密度正常组(精子密度≥20×106/ml)和少精子组(精子密度<20×106/ml);根据精子活力、活率将精子密度正常组分别分为精子活力正常组和不良组、精子活率正常组和下降组;根据精液中WBC多少将不育症患者分为WBC精液组和非WBC精液组。应用速率散射比浊法测定精浆α1-MG、IgG含量,比较分析各组间精浆α1-MG、IgG含量的差异。结果:不育组精浆IgG含量显著高于生育组(P<0.05),α1-MG含量显著低于生育组(P<0.05);精浆IgG含量在精子活力正常组明显高于活力不良组(P<0.05),在精子活率正常组明显高于活率下降组(P<0.05),在精子密度正常组与少精子组、WBC精液组和非WBC精液组之间差别无统计学意义(P>0.05);精浆α1-MG含量在WBC精液组明显高于非WBC精液组(P<0.05),在精子密度、精子活力、精子活率正常与减少组之间差异均无显著性(P>0.05);不育组精浆IgG含量与精子活力、活率呈正相关(r=0.258、0.266,均P<0.05),精浆α1-MG含量与精液WBC水平呈正相关(r=0.233,P<0.05)。结论:精浆中α1-MG、IgG含量与男性生殖关系密切,测定其含量或可为男性不育症的临床诊断、治疗及发病机制的研究提供一定的实验依据。
Objective: To observe the content of α1 microglobulin (α1-MG) and immunoglobulin G (IgG) in the seminal plasma of male infertility and its relationship with some functional indexes of semen. Methods: Semen parameters were measured in 88 cases of male infertility and 22 cases of normal fertility. According to the sperm density, the patients with infertility were divided into normal sperm density group (sperm density≥20 × 106 / ml) and oligospermia group (Sperm density <20 × 106 / ml). According to sperm motility and viability, the sperm densities of normal group were divided into normal sperm motility group and poor sperm motility group, normal sperm motility group and descending group respectively. Patients were divided into WBC semen group and non-WBC semen group. The contents of α1-MG and IgG in seminal plasma were measured by speed nephelometry. The differences of α1-MG and IgG in seminal plasma were compared between groups. Results: The content of IgG in the seminal plasma of infertile group was significantly higher than that of the reproductive group (P <0.05), and the content of α1-MG was significantly lower than that of the reproductive group (P <0.05) (P <0.05). There was no significant difference in sperm motility between normal group and active group (P <0.05), but there was no significant difference between sperm density normal group and oligospermia group, WBC semen group and non-WBC semen group (P> 0.05). The content of seminal plasma α1-MG in WBC semen group was significantly higher than that in non-WBC semen group (P <0.05), and there was no significant difference between sperm density, sperm motility, sperm motility and normal group (P> 0.05). The content of seminal plasma IgG was positively correlated with sperm motility and viability (r = 0.258,0.266, P <0.05). The content of seminal plasma α1-MG was positively correlated with semen WBC , P <0.05). CONCLUSION: The content of α1-MG and IgG in seminal plasma is closely related to male reproductive system. It may provide some experimental evidences for the clinical diagnosis, treatment and pathogenesis of male infertility.