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目的:探讨梗阻性胆管炎大鼠细胞免疫功能降低的发生机制及清热通下中药锦红片的影响.方法:♂SD大鼠24只建立急性梗阻性胆管炎模型,随机分为模型组(n=8)、锦红片治疗组(n=8)和单纯胆管梗阻组(n=8),检测血浆IL-2,CD_3~+,CD_4~+,CD_8~+,内毒素,胸腺指数,胸腺细胞凋亡指数及电镜下观察胸腺的超微结构及凋亡.结果:模型组IL-2,CD_3~+,CD_4~+和胸腺指数显著低于治疗组和单纯胆管梗阻组(IL-2:28.5±3.0 ng/L vs 33.9±3.6 ng/L,39.6±2.2 ng/L,P<0.05,P<0.01;CD_3~+:54.5%±5.5% vs 70.7%±4.8%,66.3%±7.1%,均P<0.01;CD_4~+:34.5%±8.3% vs 44.2%±3.3%,44.5%±4.2%,均P<0.01:胸腺指数:0.89±0.18 vs 1.10±0.13.1.12±0.24,均P<0.05),CD_8~+3组间没有统计学差异,血浆内毒素和凋亡指数明显高于治疗组和梗阻组(内毒素:0.85±0.14 Eu/mL vs 0.53±0.10 EU/mL,0.49±0.11 EU/mL,均P<0.01;凋亡指数:25.7±5.1 vs 15.8±5.5.9.0±3.1.P<0.05,P<0.01),模型组胸腺可见较多典型的凋亡细胞,结果显示经中药干预治疗后,免疫功能、内毒素血症和胸腺细胞凋亡有所改善,接近单纯胆管梗阻组水平.结论:梗阻性胆管炎大鼠存在免疫功能降低,胸腺细胞异常凋亡.锦红片对维持免疫机能的稳定有积极的意义.
OBJECTIVE: To investigate the mechanism of cellular immune dysfunction in rats with obstructive cholangitis and the effect of traditional Chinese medicine Jinhong Tablets. Methods: Twenty-four SD rats were established acute obstructive cholangitis and randomly divided into model groups (n = 8), Jinhong tablets treatment group (n = 8) and simple biliary obstruction group (n = 8), detection of plasma IL-2, CD_3 ~ +, CD_4 ~ +, CD_8 ~ +, endotoxin, thymus index, thymus The apoptotic index and electron microscopy were used to observe the ultrastructure and apoptosis of thymus. Results: The IL-2, CD3+, CD4+ and thymus index of the model group were significantly lower than those of the treatment group and the simple biliary obstruction group (IL-2: 28.5±3.0 ng/L vs 33.9±3.6 ng/L, 39.6±2.2 ng/L, P<0.05, P<0.01; CD_3+: 54.5%±5.5% vs 70.7%±4.8%, 66.3%±7.1% , P<0.01; CD_4~+: 34.5%±8.3% vs 44.2%±3.3%, 44.5%±4.2%, all P<0.01: thymus index: 0.89±0.18 vs 1.10±0.13.1.12±0.24, all P <0.05), There was no statistical difference between the CD8+3 groups, plasma endotoxin and apoptotic index were significantly higher than the treatment and obstruction groups (endotoxin: 0.85±0.14 Eu/mL vs 0.53±0.10 EU/mL, 0.49± 0.11 EU/mL, all P <0.01; apoptotic index: 25.7 ± 5.1 vs 15.8 ± 5.5.9.0 ± 3.1. P <0.05, P <0.01), the model group showed more typical thymus The results of the cells showed that immune function, endotoxemia, and thymocyte apoptosis were improved after interventional treatment with traditional Chinese medicine, which was close to the level of simple biliary obstruction. Conclusion: Obstructive cholangitis rats have reduced immune function and abnormal thymus cells Death. Jinhong tablets have positive significance for maintaining the stability of immune function.