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This paper presents experimental results on the effect of alkalis such as NaOH, KOH and Ca(OH)2 on deacetylation of a konjac glucomannan (KGM) powder under mechano-chemical (MC) treatment. The results show that the alkalinity of modifiers is a dominant factor for deacetylation of KGM. In addition,characteristics, such as swelling property, viscous stability and thermal behavior of the deacetylated KGM are analyzed.