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通过原核表达系统表达猪传染性胃肠炎病毒( TGEV) S基因不同编码区,以鉴定不同编码区表达产物与TGEV抗体的结合能力。在对TGEV S蛋白抗原位点分析的基础上,针对S蛋白N端的4个抗原位点C、B、D和A,设计引物分别扩增含C、B位点的TCB片段(411 bp)、含D位点的TD片段(441 bp)和含A位点的TA片段(456 bp),经克隆测序后分别插入原核表达载体pET-32a中进行表达,SDS-PAGE电泳显示各片段均高效表达,表达的TCB片段、TD片段和TA片段融合蛋白大小分别为34.6,35.1,36.1 kDa。 Western Blot结果表明,表达蛋白与TGEV感染猪阳性血清和His标签抗体均产生阳性反应。结果显示,3个S基因编码片段均具有与TGEV抗体结合的能力,为进一步鉴定不同编码区的免疫原性及抗原保护能力奠定了基础。“,”To identify immunoreactivity of different encoding regions of the spike ( S ) gene of Transmissible gastroenteritis virus ( TGEV) ,three encoding regions containing four antigenic sites C,B,D and A located at the N-terminus of S protein was analyzed and expressed by prokaryotic expression system. The fragments TCB ( containing antigenic sites C and B,411 bp) ,TD ( containing antigenic site D,441 bp) and TA ( containing antigenic site A, 456 bp) were amplified by PCR. After cloning and sequence,the fragments were inserted into the prokaryotic ex-pression vector pET-32a respectively. The fusion proteins yielded by TCB,TD and TA recombinant expression plas-mids were 34. 6,35. 1,36. 1 kDa respectively. The expressed proteins showed strong positive reaction with TGEV-infected swine serum and His tag antibody by Western Blot. These results indicated that the three encoding regions of S gene had a strong immunoreactivity with TGEV positive antiserum,which will play an essential role in identif-ying immunogenicity and immunological protection of different encoding regions of the S protein of TGEV.