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目的了解狂犬病毒在疫区犬间的流行状况。方法收集浙江省丽水市198份狂犬病新发疫区犬脑组织标本,用直接免疫荧光试验(DFA)特异性检测狂犬病毒抗原和逆转录-聚合酶链反应(RT-PCR)特异性检测狂犬病毒核酸,确定犬感染狂犬病毒状况。结果犬脑组织DFA和RT-PCR平行检测阳性的标本13份,阳性率为6.57%(13/198),其中松阳县9份,占采集标本的69.23%(9/13),具有攻击人/犬史或者被犬攻击史的疑似狂犬病犬标本10份,占采集标本的76.92%(10/13)。结论免疫学和分子生物学两种方法平行进行犬狂犬病毒的实验室检测,可以更准确地进行狂犬病的实验室诊断。市民暴露于犬后要及时到所在地狂犬病暴露门诊严格按照国家相关标准及规范正确处理伤口并注射狂犬病疫苗及抗血清或人源免疫球蛋白。建议相关部门加强犬的管理,同时对犬进行全面的狂犬病疫苗免疫接种。
Objective To understand the prevalence of rabies virus in canine dogs. Methods A total of 198 rabies and canine newly diagnosed canine brain tissues were collected from Lishui City, Zhejiang Province. The rabies virus antigen was detected by direct immunofluorescence assay (DFA) and reverse transcription-polymerase chain reaction (RT-PCR) Nucleic acids to determine the status of rabies infection in dogs. Results Thirteen positive samples were detected by DFA and RT-PCR. The positive rate was 6.57% (13/198), of which 9 were in Songyang County, accounting for 69.23% (9/13) of the collected samples, / Dog or history of dog attack rabies suspected rabies specimens 10, accounting for 76.92% (10/13) collected specimens. Conclusions Both immunological and molecular biological methods can be used for parallel testing of canine rabies virus in laboratory, which can make laboratory diagnosis of rabies more accurate. Public exposure to dogs should be promptly to the local rabies exposure clinics in strict accordance with national standards and norms to properly handle the wounds and injection of rabies vaccine and antiserum or human immunoglobulin. Recommendations related departments to strengthen the management of dogs, dogs at the same time a comprehensive rabies vaccine immunization.