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目的甲型H1N1流感病毒A/California/7/2009与A/California/4/2009病毒序列比较同源性在99%以上,本实验旨在比较两株病毒感染BALB/c小鼠研究感染力强弱。方法分别将A/California/7/2009(CA7)与A/California/4/2009(CA4)两株病毒分别连续10倍稀释后,对4~6周龄雌性BALB/c小鼠经乙醚麻醉后进行滴鼻攻毒,每个稀释度接种10只实验小鼠,测定CA7 MLD50为101.24/0.05 mL,检测小鼠感染、致病的多项指标,观察期为14 d。结果相同TCID50的CA7和CA4病毒感染小鼠,CA4感染小鼠后14 d内死亡率为20%,而CA7感染小鼠后8 d内死亡率为100%。CA7 106TCID50感染的小鼠病理表现为重度弥漫性间质性肺炎,CA4 106TCID50感染的小鼠病理表现为中度-重度间质性肺炎。结论在相同条件下,CA7感染力明显强于CA4。
Objective To compare the homology between the A / California / 7/2009 and A / California / 4/2009 virus sequences of A / California / 7/2009 with more than 99% homology. The aim of this study was to compare the infectivity of two strains of virus infected BALB / c mice weak. Methods The female BALB / c mice of 4 ~ 6 weeks old were anesthetized with diethyl ether after diluting the two viruses of A / California / 7/2009 (CA7) and A / California / 4/2009 The mice were inoculated intranasally and inoculated with 10 experimental mice at each dilution. The CA7 MLD50 was determined to be 101.24 / 0.05 mL. Multiple indicators of infection and pathogenicity were detected in mice. The observation period was 14 days. Results In mice infected with CA7 and CA4 viruses of the same TCID50, the mortality rate was 20% within 14 days after CA4 infection in mice, and 100% in mice infected with CA7 within 8 days. Mice infected with CA7 106TCID50 were pathologically characterized by severe diffuse interstitial pneumonia. CA4 106TCID50-infected mice were pathologically characterized by moderate-severe interstitial pneumonia. Conclusion Under the same conditions, CA7 infectivity is obviously stronger than CA4.