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目的观察酒精暴露对新生小鼠视网膜结构的影响,以及肝脏X受体(LXRs)激动剂TO901317对酒精损伤小鼠视网膜的保护作用。方法将新生C57BL/6J小鼠分为对照组、酒精暴露组和激动剂TO预处理组,每组3只。采用HE染色检测各组小鼠视网膜大体结构改变,采用免疫组化染色检测各组小鼠视网膜NeuN阳性细胞数、GFAP表达变化。结果免疫组化染色发现LXRβ主要分布于新生小鼠视网膜节细胞层,HE染色发现酒精组新生小鼠视网膜节细胞数量较对照组显著减少(P<0.01),TO处理能显著翻转酒精暴露导致的视网膜节细胞层细胞数量减少(P<0.05);免疫组化染色发现酒精组小鼠眼球视网膜节细胞层NeuN阳性细胞数明显少于对照组(P<0.01),而TO预处理组较酒精组NeuN阳性细胞显著增多(P<0.01)。对各组进行GFAP免疫组化染色并采用光密度分析进行半定量分析,结果显示酒精组小鼠视网膜GFAP表达较对照组和TO预处理组显著增高(P<0.01)。结论 LXRs受体激活可有效抑制酒精所致视网膜中星形胶质细胞的活化,挽救节细胞丢失。
Objective To observe the effects of alcohol exposure on the retina structure of newborn mice and the protective effect of hepatic X receptor (LXRs) agonist TO901317 on the retina of alcohol-injured mice. Methods Newborn C57BL / 6J mice were divided into three groups: control group, alcohol exposure group and agonist TO pretreatment group. The gross structure of the retina in each group was detected by HE staining. The number of NeuN positive cells and the expression of GFAP in the retina were detected by immunohistochemical staining. Results Immunohistochemical staining showed that LXRβ mainly distributed in the retinal ganglion cell layer of neonatal mice. HE staining showed that the number of retinal ganglion cells in neonatal mice decreased significantly (P <0.01), and TO treatment significantly reversed the alcohol exposure The number of cells in retinal ganglion cell layer decreased (P <0.05). The number of NeuN positive cells in retinal ganglion cell layer in alcohol group was significantly lower than that in control group (P <0.01) NeuN positive cells increased significantly (P <0.01). GFAP immunohistochemical staining of each group and optical density analysis of semi-quantitative analysis revealed that the expression of GFAP in alcohol group was significantly higher than that in control group and TO pretreatment group (P <0.01). Conclusion LXRs receptor activation can effectively inhibit the activation of astrocytes in the retina induced by alcohol and save the loss of ganglion cells.