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目的:探讨补阳还五汤(BYHWD)对脊髓损伤(SCI)大鼠血浆血小板活化因子(PAF)含量的影响及BYHWD的中枢神经保护作用机制。方法:采用Allen’s打击法制作中度SCI模型,应用SPF级Wistar大鼠,随机分为4组:正常组和3个模型组,即模型组、BYHWD组、金纳多组,每组16只大鼠。模型组大鼠分别给予蒸馏水、BYHWD、金纳多水溶液,正常组给予蒸馏水,灌胃治疗2周后,采用双抗体夹心法(ELISA法)检测血浆PAF含量,并在造模前至造模后3周对SCI大鼠进行运动功能评分。结果:造模1周后给药前BYHWD组、金纳多组与模型对照组动物运动功能评分显著低于正常对照组(P<0.01),但3组间差异无统计学显著性。给药后1周、2周检测结果与模型组比较,BYHWD和金纳多组运动功能评分显著高于模型组,低于正常组(P<0.01);两个给药组间差异无统计学意义(P>0.05)。造模后第3周末模型组PAF含量明显升高,BYHWD组可明显降低SCI大鼠血浆PAF含量,其抑制作用较金纳多明显,差异有显著性统计学意义(P<0.01)。结论:BYHWD可明显改善SCI大鼠后肢运动功能,减少SCI大鼠内源性PAF含量,发挥对损伤脊髓的保护作用,其降低SCI大鼠血浆中PAF含量与改善其后肢运动功能相关。
Objective: To investigate the effect of BYHWD on plasma platelet activating factor (PAF) content in rats with spinal cord injury (SCI) and the mechanism of BYHWD central nervous system protection. Methods: Allen’s hitting method was used to make moderate SCI model. SPF Wistar rats were randomly divided into 4 groups: normal group and 3 model groups, namely model group, BYHWD group, and Ginaton group, with 16 rats in each group. mouse. Rats in the model group were given distilled water, BYHWD, and Jinaluo aqueous solution respectively. Normal group was given distilled water. After 2 weeks of gavage, plasma PAF levels were measured by double-antibody sandwich method (ELISA), and before the model was established, after the model was established. SCI rats were scored for motor function at 3 weeks. RESULTS: The motor function scores of the BYHWD group, Ginaton group, and model control group before administration were significantly lower than those of the normal control group (P<0.01). However, there was no statistically significant difference among the three groups. The results of the 1 week and 2 weeks after administration were compared with the model group. The scores of motor function in BYHWD and Ginaton group were significantly higher than those in the model group and lower than the normal group (P<0.01). There was no statistical difference between the two groups. Significance (P>0.05). At the end of the third week after model establishment, the PAF content in the model group increased significantly. The BYHWD group could significantly reduce the plasma PAF content in the SCI rats, and the inhibitory effect was significantly higher than that of Ginaton. The difference was statistically significant (P<0.01). Conclusion: BYHWD can significantly improve the motor function of hindlimbs in SCI rats, reduce the endogenous PAF content in SCI rats, and play a protective role in spinal cord injury. The decrease of PAF in plasma of SCI rats is related to the improvement of hindlimb motor function.