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目的 :探讨脾虚证大鼠甘草甜素 (GL)的药物动力学 (PK)特征 ,并从胃排空、脑 肠肽两方面探讨其机制。方法 :利血平作用于大鼠 14d ,复制实验性脾虚模型 (C组 ) ;用四君子汤 (A组 )和六味地黄汤 (B组 )治疗以反证 ;用高效液相色谱 (HPLC)法测定大鼠体内GL的血药浓度 ;用同位素检测其胃液体排空功能 ;用放射免疫分析法 (RIA)分析下丘脑和血浆中胃动素 (MOT)、胆囊收缩素 (CCK)及生长抑素 (SS)的浓度。结果 :GL在体内为二室房室PK模型。与正常对照组 (D组 )比较 ,C组大鼠药物动力学相关参数降低 ;胃排空率下降 ,MOT和SS在血浆及下丘脑的水平下降 ,而CCK则上升 ;A组上述各值恢复正常 ,与D组比较无统计学差异 (P >0 .0 5) ;B组与A组比较仍有显著差异 (P <0 .0 5,P <0 .0 1)。结论 :脾虚大鼠胃排空、脑 肠肽浓度的异常 ,与脾虚大鼠GLPK特征的异常密切相关
Objective: To investigate the pharmacokinetic (PK) characteristics of glycyrrhizin (GL) in spleen deficiency rats and to explore its mechanism from gastric emptying and brain gut peptides. METHODS: Reserpine was applied on rats for 14 days, and experimental spleen deficiency model (C group) was duplicated. Treatment with Sijunzi Decoction (group A) and Liuwei Dihuang Decoction (group B) was used as anti-evidence; and it was determined by high performance liquid chromatography (HPLC). Plasma concentration of GL in rats; determination of gastric emptying by isotopes; analysis of motilin (MOT), cholecystokinin (CCK), and somatostatin in the hypothalamus and plasma by radioimmunoassay (RIA) (SS) concentration. RESULTS: GL was a two-compartment atrioventricular PK model in vivo. Compared with normal control group (D group), pharmacokinetic parameters of group C rats decreased; gastric emptying rate decreased, MOT and SS decreased in plasma and hypothalamus, and CCK increased; group A above values recovered Normally, there was no statistical difference between group D and group D (P > 0.05). There was still significant difference between group B and group A (P <0.05, P <0.01). Conclusion: Abnormalities of gastric emptying and brain-gut peptide concentration in spleen deficiency rats are closely related to abnormal GLPK characteristics in spleen-deficit rats.